组织工程与重建外科杂志2025，Vol.21Issue(6)：561-570,10.DOI:10.3969/j.issn.1673-0364.2025.06.007
基于DLP的3D打印仿生支架负载ADSC-EVs修复长骨缺损的实验研究
Experimental research of long bone defect repair using biomimetic scaffold constructed via DLP 3D printing loaded with ADSC extracellular vesicles
摘要
Abstract
Objective To investigate the reparative effect of 3D printed biomimetic bone scaffolds loaded with adipose stem cell-derived extracellular vesicles(ADSC-EVs)on long bone defects.Methods High-precision biomimetic bone scaffolds were fabricated using digital light processing(DLP)3D printing with polycaprolactone triacrylate(PCLTA).ADSC-EVs were drop-loaded onto the scaffold surface.Scanning electron microscopy was used to observe the morphology of the vesicles on the scaffold.Cell compatibility and osteogenic ability of the scaffold were evaluated using immunofluorescence staining with phalloidin and Alizarin Red.Critical bone defects(15 mm)were created in the bilateral radius of New Zealand white rabbits.Rabbits were randomly assigned to 3 groups:blank control group without scaffold implantation,control group with pure material scaffold implantation,and experimental group with scaffold loaded with ADSC-EVs.Samples were harvested at 12 weeks post-implantation for evaluation of osteogenesis and angiogenesis using X-ray,immunohistochemical staining,and histological analysis.Results Phalloidin staining showed uniform cell growth on the scaffold surface with normal cell morphology.Alizarin Red staining revealed that the vesicle-loaded scaffold had stronger osteoinductive ability(P<0.05).Scanning electron microscopy demonstrated uniform distribution of vesicles on the scaffold surface and within the scaffold.X-ray images indicated that the experimental group had faster new bone formation and higher bone mass than the control group(P<0.05)and blank control group(P<0.01),which was further confirmed by histological analysis with Hematoxylin-Eosin staining.Immunohistochemical staining of tissue sections showed that the expression of the endothelial cell-specific marker CD31 was higher in the experimental group than in the control group(P<0.05)and blank control group(P<0.01).Conclusion PCLTA biomimetic bone scaffolds loaded with ADSC-EVs exhibit excellent osteogenic and angiogenic abilities,effectively promoting the repair of long bone defects.关键词
数字光处理/仿生骨支架/细胞外囊泡/长骨再生Key words
Digital light processing/Biomimetic bone scaffold/Extracellular vesicles/Long bone regeneration分类
医药卫生引用本文复制引用
姜文彬,任金珍,张一帆,陈雳风..基于DLP的3D打印仿生支架负载ADSC-EVs修复长骨缺损的实验研究[J].组织工程与重建外科杂志,2025,21(6):561-570,10.基金项目
国家自然科学基金(82002067) （82002067）
中国博士后科学基金(2021M690063). （2021M690063）
