中国现代医生2025，Vol.63Issue(34)：1-7,7.DOI:10.3969/j.issn.1673-9701.2025.34.001
基于生物信息学分析鉴定氟中毒诱导重度抑郁症的生物标志物及原花青素拮抗作用
Biomarkers of severe depression induced by fluorosis and the antagonistic effect of proanthocyanidins were identified based on bioinformatics analysis
摘要
Abstract
Objective To explore the potential mechanism of action of genes related to severe depression induced by fluoride and the alleviating effect of proanthocyanidin(PC).Methods The datasets of glial cells exposed to fluoride and the genetic data of patients with depression were obtained from the Gene Expression Omnibus.Analyze the differential expressed genes(DEG),and screen out the key genes through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis and protein-protein interaction(PPI)analysis.And construct the key gene-microRNA(miRNA)and key gene-transcription factor(TF)networks,and then carry out molecular docking of ligands with key targets.The expression of poly adenosine diphosphate-ribose polymerase(PARP)1 and cleaved-PARP in SH-SY5Y cells was detected by Western blotting.Results A total of 95 common DEGs were screened out,mainly enriched in pathways such as apoptosis.Three key genes(UQCRQ,SNRPD2 and TIMM 10)were screened out.Receiver operating characteristic curve analysis showed that TIMM 10 had the strongest predictive ability for depression.The key gene-miRNA network indicated that five miRNAs jointly regulate these three key genes.The key gene-TF network concluded that ELF1 regulated these three key genes.Molecular docking indicated that the UQCRQ receptor had a high affinity for the PC ligand.The results of in vitro experiments showed that the expression of PARP1 in NaF group was lower than that in control group(P＜0.05),while the expression of PARP1 in NaF+PC group was higher than that in NaF group(P＜0.05).The expression of cleaved-PARP in NaF group was significantly higher than that in control group(P＜0.05),and the expression of cleaved-PARP in NaF+PC group was significantly lower than that in NaF group(P＜0.05).Conclusion PC can effectively alleviate severe depression caused by fluoride and has a certain protective effect on depression.关键词
生物信息学/氟中毒/重度抑郁症/原花青素Key words
Bioinformatics/Fluorosis/Severe depression/Proanthocyanidin分类
医药卫生引用本文复制引用
肖梦欢,吴志宇,颜楚麟,牛强,马娇龙,郭淑霞..基于生物信息学分析鉴定氟中毒诱导重度抑郁症的生物标志物及原花青素拮抗作用[J].中国现代医生,2025,63(34):1-7,7.基金项目
国家自然科学基金项目(82360671,82060580) （82360671,82060580）
