中药新药与临床药理2025，Vol.36Issue(12)：2080-2090,11.DOI:10.19378/j.issn.1003-9783.2025.12.008
基于人用经验、网络药理学和分子对接探讨鸡血藤治疗重症肌无力的作用机制
Exploring the Mechanism of Spatholobi Caulis in Treating Myasthenia Gravis Based on Human Experience,Network Pharmacology,and Molecular Docking
摘要
Abstract
Objective To investigate the mechanism of the effective single herb Spatholobi Caulis in treating myasthenia gravis(MG)based on human experience,network pharmacology,and molecular docking.Methods Active components of Spatholobi Caulis and their targets were screened using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).MG-related targets were obtained from GeneCards and OMIM databases.After unifying target names with Uniprot,intersection targets were identified for subsequent analysis.A drug component-target network was constructed using Cytoscape 3.7.1,and a protein-protein interaction(PPI)network was established based on the STRING platform.GO annotation and KEGG pathway enrichment analyses were performed using DAVID.Molecular docking verification of active components with core targets was completed using AutoDock Vina.Results A total of 23 active compounds(such as luteolin)and 128 action targets were screened from Spatholobi Caulis.There were 1 115 disease targets corresponding to MG,with 31 shared targets between Spatholobi Caulis and MG.Thirteen core targets were identified,among which acetylcholinesterase(AChE)showed the highest Node Stress value.Enrichment analysis revealed that Spatholobi Caulis might intervene in the pathogenesis of MG by regulating 154 signaling pathways(such as endocrine resistance and AGE-RAGE signaling pathway).These core targets and pathways participate in MG-related pathological mechanisms,including abnormal AChE activity,neuromuscular junction damage,altered synaptic plasticity,macrophage phagocytosis of acetylcholine receptor(AChR),impaired apoptosis of autoreactive lymphocytes,immune tolerance defects,and endocrine resistance.Molecular docking results indicated that luteolin exhibited the highest binding affinity with AChE,followed by formononetin and calycosin binding to AChE.Conclusion Spatholobi Caulis may treat MG by regulating AChE activity,modulating autophagy processes,promoting apoptosis of autoreactive lymphocytes,and reducing endocrine resistance.Luteolin contained in Spatholobi Caulis can reduce anti-AChR antibodies,inhibit AChE activity,and decrease macrophage phagocytosis of AChR,showing high development value for treating MG accompanied by memory impairment.However,the use of Spatholobi Caulis for MG with thymoma remains controversial and warrants further discussion.关键词
重症肌无力/鸡血藤/木犀草素/人用经验/网络药理学/分子对接/乙酰胆碱酯酶/神经肌肉接头Key words
myasthenia gravis/Spatholobi Caulis/luteolin/human experience/network pharmacology/molecular docking/acetylcholinesterase/neuromuscular junction分类
医药卫生引用本文复制引用
张驰名,何星沂,戚真榕,陈凯佳,刘小斌..基于人用经验、网络药理学和分子对接探讨鸡血藤治疗重症肌无力的作用机制[J].中药新药与临床药理,2025,36(12):2080-2090,11.基金项目
广东省医学科学技术研究基金项目(B2021155) （B2021155）
第七批"全国老中医药专家学术经验继承工作"项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]239号) （国中医药人教函[2022]239号）
刘小斌省名中医传承工作室建设项目(粤中医办函[2023]108号) （粤中医办函[2023]108号）
广州中医药大学揭榜挂帅项目(广中医校办[2024]163号). （广中医校办[2024]163号）
