中药材2025，Vol.48Issue(4)：848-853,6.DOI:10.13863/j.issn1001-4454.2025.04.008
多重荧光PCR法鉴别半夏药材及饮片中的掺混伪品
Development and Application of Multiplex Fluorescent PCR for Identification of Pinellia ternate from its Counterfeits
摘要
Abstract
Objective:To establish a multiplex fluorescent PCR method for identification of Pinellia ternate and its common counter-feits Pinellia pedatisecta,Typhonium flagelliforme,Arisaema erubescens,and to verify its feasibility,so as to provide methodological support for formulating the quality standard of Pinellia ternate.Methods:The multiplex PCR system was optimized,and the specificity,sensitivity,detection limit of the method were investigated.The 15 batches of medicinal materials and decoction pieces suspeeted to be Pinellia ternate counterfeit in the earlier stage were tested and verified.Results:The method established in this study detected 8 batches of Pinellia pedati-secta,3 batches of Typhonium flagelliforme and 4 batches of non-Pinellia ternate other confounding products,and the measured results were completely consistent with the sequencing results.The method had no cross-reaction in the common related species of the same family,and only needed the template amount of pg DNA to detect,and could detect the mixed counterfeits with only 5%adulteration rate.Con-clusion:The established multiplex fluorescent PCR method has the advantages of sensitivity,speed and accuracy,and can be widely used as a specific detection method for Pinellia ternate medicinal materials and decoction pieces and its common counterfeits.关键词
半夏/虎掌/水半夏/天南星/多重荧光PCRKey words
Pinellia ternate(Thunb.)Breit./Pinellia pedatisecta Schott/Typhonium flagelliforme(Lodd.)Blume/Arisaema erubescens(Wall.)Schott/Multiplex fluorescent PCR分类
医药卫生引用本文复制引用
张聪聪,高鹏,伍欣然,毛腾霄,程龙,陈蓉..多重荧光PCR法鉴别半夏药材及饮片中的掺混伪品[J].中药材,2025,48(4):848-853,6.基金项目
国家自然科学基金项目(81973434) （81973434）
