作物学报2026，Vol.52Issue(1)：28-43,16.DOI:10.3724/SP.J.1006.2026.51069
藜麦RopGEF家族基因的鉴定及表达模式分析
Identification and expression pattern analysis of RopGEF family genes in Chenopodium quinoa
摘要
Abstract
In plants,RopGEF-mediated ROP signaling plays a crucial role in cellular signaling pathways.In this study,seven RopGEF family members were identified in Chenopodium quinoa through bioinformatics analysis and were found to be distrib-uted across six chromosomes.Based on phylogenetic relationships and structural characteristics,90 RopGEFs from six crop spe-cies,including Arabidopsis thaliana and rice,were classified into four subfamilies.Evolutionary analysis revealed that CqRop-GEF5 is closely related to AtRopGEF1 and OsRopGEF1.Structural analysis indicated that the exon-intron organization,protein motif composition,and secondary and three-dimensional structures of CqRopGEFs are highly conserved.qRT-PCR analysis showed that most CqRopGEFs were highly expressed during seed germination,with expression levels in seedling roots being higher than in stems and leaves.Their expression was significantly induced by exogenous abscisic acid(ABA)and abiotic stresses.For instance,the expression of CqRopGEF2,CqRopGEF3,CqRopGEF4,and CqRopGEF7 initially increased and then decreased under ABA treatment,while CqRopGEF7 was strongly downregulated under both cold and heat stress.In conclusion,the CqRopGEF gene family exhibits conserved evolutionary structure and may play important roles in quinoa growth and develop-ment,ABA signaling,and responses to abiotic stress.关键词
藜麦/RopGEFs/系统进化分析/基因表达/非生物胁迫Key words
Chenopodium quinoa/RopGEFs/phylogenetic analysis/gene expression/abiotic stresses引用本文复制引用
景秀清,蔡永朵,邓宁,赵晓东,翟飞红,曾群..藜麦RopGEF家族基因的鉴定及表达模式分析[J].作物学报,2026,52(1):28-43,16.基金项目
本研究由山西省基础研究计划(自由探索类)项目(202203021212186),太原师范学院 2024年度研究生教育创新项目(SYYJSYC-2431)和太原师范学院大学生创新创业训练项目(CXCY25104)资助. This study was supported by the Fundamental Research Programs of Shanxi Province(202203021212186),the Graduate Education and In-novation Projects of Taiyuan Normal University(SYYJSYC-2431),and the College Student Innovation and Entrepreneurship Training Pro-gram of Taiyuan Normal University(CXCY25104). （自由探索类）
