作物学报2026，Vol.52Issue(1)：44-55,12.DOI:10.3724/SP.J.1006.2026.53046
植物胆碱单加氧酶基因家族的进化与表达分析
Evolution and expression analysis of the choline monooxygenase gene family in plants
摘要
Abstract
Glycine betaine(GB)is a compatible solute widely found in plants,animals,bacteria,and algae,where it plays a key role in regulating osmotic pressure and maintaining cellular water balance upon accumulation in the cytoplasm.As such,GB is crucial for plant responses to osmotic stress.Choline monooxygenase(CMO)is a rate-limiting enzyme in the biosynthetic path-way of GB in plants.Among 168 genomes analyzed in this study,131 were found to contain homologous CMO genes,with a total of 169 CMO members identified.Selective pressure analysis revealed that most gene pairs have undergone purifying selection,while only seven gene pairs exhibited signs of positive selection.Phylogenetic analysis classified the CMO gene family into six subfamilies,with positive selection sites detected in five subfamilies except subfamily F.Further analysis of the Rieske domain and Ring-hydroxyl A domain showed a significant positive correlation between their dN/dS values,suggesting co-evolution.qRT-PCR analysis demonstrated that the expression of CMO genes in maize was upregulated under MgSO4-induced stress.This study provides a comprehensive analysis of the evolution and expression of the CMO gene family,offering a theoretical founda-tion for the future application of CMO genes in improving crop tolerance to abiotic stress.关键词
植物甘氨酸甜菜碱/胆碱单加氧酶基因/选择压/纯化选择/玉米Key words
plant glycine betaine/choline monooxygenase gene/selection pressure/purifying selection/maize引用本文复制引用
王婷,段武丽,王蕊,刘海岚..植物胆碱单加氧酶基因家族的进化与表达分析[J].作物学报,2026,52(1):44-55,12.基金项目
本研究由国家自然科学基金项目(32271984)资助. This study was supported by the National Natural Science Foundation of China(32271984). （32271984）
