中医康复2026，Vol.3Issue(1)：64-71,8.DOI:10.19787/j.issn.2097-3128.2026.01.010
超短波对急性肺损伤大鼠炎性反应及肺泡上皮细胞凋亡的影响
Effects of Ultrashort Waves on Inflammatory Response and Alveolar Epithelial Cell Apoptosis in Rats with Acute Lung Injury
摘要
Abstract
Objective:To investigate the effects of ultrashort waves on anti-inflammatory capacity and cell apoptosis in LPS-induced acute lung injury rats.Methods:Thirty male SD rats were randomly divided into the control group,the LPS injury group and the ultrashort wave group,with 10 rats in each group.The acute lung injury model was established by lipopolysaccharide tail vein injection,and the ultrashort wave group was given ultrashort wave intervention for 15 min immediately,24 h and 48 h after modelling;the control group and the LPS injury group were placed in the middle of the unelectrified ultrashort wave electrode plate.Immediately after the intervention,the rats were put to death,the expression of IL-1β and IL-10 in serum was detected by ELISA,the degree of lung injury was assessed by HE staining,the apoptotic changes of alveolar epithelial cells were detected by TUNEL,and the expression of caspase-3 and caspase-8 mRNA and protein were detected by PCR and Western-blot,respectively.Results:Compared with the control group,serum IL-1β expression was significantly higher and IL-10 expression was significantly lower in the LPS-injured group(P<0.01),lung tissue structure was significantly damaged,lung injury score and apoptosis rate of alveolar epithelial cells were significantly higher(P<0.01),and the expression of caspase-3 and caspase-8 mRNA and protein was significantly higher(P<0.01).After ultrashort wave intervention,LPS-injured rats showed a significant decrease in serum IL-1β expression,a significant increase in IL-10 expression(P<0.01),a significant decrease in lung injury score and apoptosis rate of alveolar epithelial cells(P<0.01),and a significant decrease in caspase-3 and caspase-8 mRNA and protein expression(P<0.01).Conclusion:Ultrashort wave therapy exerts anti-inflammatory effects on lipopolysaccharide-induced acute lung injury by inhibiting alveolar epithelial cell apoptosis by downregulating the caspase-8/caspase-3/IL-1β signaling pathway.关键词
超短波/急性肺损伤/炎性反应/caspase-3/caspase-8/细胞凋亡Key words
ultrashort wave therapy/acute lung injury/inflammatory response/caspase-3/caspase-8/apoptosis分类
医药卫生引用本文复制引用
WEN Xing,JIA Feiyang,LI Mengmeng,LIU Yuan,NING Pengyun,ZENG Yahua,HUANG Xiarong,LIU Haidi,ZHOU Jun..超短波对急性肺损伤大鼠炎性反应及肺泡上皮细胞凋亡的影响[J].中医康复,2026,3(1):64-71,8.基金项目
湖南省自然科学基金(2023JJ40586) （2023JJ40586）
湖南省卫健委项目(202218014485,B202314058540) （202218014485,B202314058540）
南华大学附属第一医院4310计划(20214310NHYCG07) （20214310NHYCG07）
