重庆大学学报2025，Vol.48Issue(12)：20-32,13.DOI:10.11835/j.issn.1000-582X.2024.265
聚苯乙烯纳米塑料和Cu2+对施氏假单胞菌的复合胁迫研究
Combined stress effects of polystyrene nanoplastics and Cu2+on Pseudomonas stutzeri
摘要
Abstract
The coexistence of polystyrene nanoplastics(PS-NPs)and Cu2+in wastewater can induce compound stress effects on microorganisms.This study investigated the effects of PS-NPs and Cu2+on the growth,nitrogen removal,and biochemical characteristics of the aerobic denitrifying bacterium Pseudomonas stutzeri,and further elucidated the stress mechanism through transcriptomics analysis.The results showed that under exposure to 50 mg/L PS-NPs,bacteria growth activity increased by 25.3%compared to the control,accompanied by enhanced nitrogen removal and significant upregulation of genes related to ribosome function,the tricarboxylic acid(TCA)cycle,and ABC transport pathways.In contrast,under 10 mg/L Cu2+stress,bacterial growth activity decreased by 83.2%compared to the control,with severe inhibition,cell membrane damage,and marked downregulation of ribosomal,nitrogen metabolism,and ABC transport pathways.Genes related to extracellular polymeric substances(EPS)secretion and cell membrane synthesis were significantly upregulated.Under combined stress,Cu2+exerted the dominant inhibitory effect;however,PS-NPs promoted EPS secretion,which,along with PS-NPs themselves,adsorbed part of the Cu2+ions and mitigated their toxicity.关键词
好氧反硝化菌/聚苯乙烯纳米塑料/铜离子/胁迫/转录组学Key words
aerobic denitrifying bacteria/polystyrene nanoplastics/copper ion/combined stress/transcriptomics分类
资源环境引用本文复制引用
MENG Weile,QU Jianwei,HUANG Jie,YANG Rui,XU Xiaowei,CHEN Youpeng,YAN Peng,FANG Fang,GUO Jinsong..聚苯乙烯纳米塑料和Cu2+对施氏假单胞菌的复合胁迫研究[J].重庆大学学报,2025,48(12):20-32,13.基金项目
国家博士后研究人员计划(GZC20233319) （GZC20233319）
国家自然科学基金项目(42207022,51878091). Supported by the National Postdoctoral Research Program(GZC20233319),and National Natural Science Foundation of China(42207022,51878091). （42207022,51878091）
