肝胆胰外科杂志2025，Vol.37Issue(12)：822-831,10.DOI:10.11952/j.issn.1007-1954.2025.12.006
IL-27通过调节巨噬细胞极化状态治疗急性胰腺炎的实验研究
Experiment research of IL-27 in the treatment of acute pancreatitis by regulating the polarization of macrophages
LI Ying 1YU Haohui 1CHEN Ge'er 1WANG Junru 1ZHOU Shiyu 1HUANG Longyue 1SUN Hongwei1
作者信息
- 1. @@@aDepartment of Operating Room,bDepartment of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325000,China
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摘要
Abstract
Objective To investigate the role of interleukin-27(IL-27)in inducing macrophage polarisation towards the M2 type and thus exerting a therapeutic effect in acute pancreatitis(AP).Methods To compare the changes of inflammatory factors in AP mice.The cell model of acute pancreatitis was established by using INF-γ(500 U/mL)+LPS(100 ng/mL),which induced the polarisation of RAW264.7 macrophages to M1 type,and then co-culturing the RAW264.7 macrophages overexpressing IL-27 with them to observe whether the macrophages were polarised from M1 to M2 type,and to detect the related changes of inflammatory factors.Finally,the above related molecular mechanisms were preliminary explored by ITRAQ quantitative proteomics assay.Results In AP mice,IL-27 expression was reduced and other pro-inflammatory factors were significantly overexpressed.After transfection of RAW264.7 macrophages with IL-27,the cell viability of RAW264.7 macrophages was completely unaffected,the expression of the M1 marker CD86 was significantly decreased(P<0.01),and the expression of the M2 marker CD206 was significantly increased(P<0.05).M1 macrophages were significantly increased after local inflammatory stimulation,but the expression of CD86 was greatly reduced(P<0.05),and the expression of the inflammatory factor TNF-ɑ was also significantly decreased(P<0.01)after co-culture of macrophages transfected with IL-27.ITRAQ quantitative proteomics results indicated that the above effects may be related to the NF-κB and PI3K/AKT signaling pathways,and further Western blotting results were consistent with the proteomics results.Conclusion IL-27 induces the polarisation of RAW264.7 macrophages from M1-type to M2-type via the NF-ĸB and PI3K/AKT pathways,which reduces the inflammatory response and achieves the therapeutic effect in acute pancreatitis.关键词
急性胰腺炎/白细胞介素-27/巨噬细胞/极化状态/核因子κBKey words
acute pancreatitis/interleukin-27/macrophage/polarized state/nuclear factor kappa B分类
医药卫生引用本文复制引用
LI Ying,YU Haohui,CHEN Ge'er,WANG Junru,ZHOU Shiyu,HUANG Longyue,SUN Hongwei..IL-27通过调节巨噬细胞极化状态治疗急性胰腺炎的实验研究[J].肝胆胰外科杂志,2025,37(12):822-831,10.
