保健医学研究与实践2025，Vol.22Issue(10)：21-27,7.DOI:10.11986/j.issn.1673-873X.2025.10.04
胃癌组织中TDIF1、miR-452-5p表达水平与临床病理特征及预后的关系
Expression of TDIF1 and miR-452-5p in gastric cancer tissue and their relationship with clinical pathological features and prognosis
摘要
Abstract
Objective To explore the expression levels of TdT-interacting factor 1(TDIF1)and microRNA-452-5p(miR-452-5p)in gastric cancer tissue and their relationship with clinical pathological features and prognosis in gastric cancer patients.Methods This study included 90 gastric cancer patients who underwent surgery at the First Affiliated Hospital of Xi'an Jiao-tong University from May 2018 to May 2021.Tumor tissue and normal gastric mucosal tissue at least 5 cm away from the tumor margin were collected during surgery.The relative expression levels of TDIF1 and miR-452-5p in these tissues were de-tected using quantitative real-time PCR(qRT-PCR).Pearson correlation analysis was performed to assess the correlation be-tween TDIF1 and miR-452-5p expression in gastric cancer tissue.Kaplan-Meier survival curves were used to analyze the rela-tionship between their expression levels and patient prognosis.Cox regression analysis was used to identify the factors affecting the prognosis of gastric cancer patients.Results The expression levels of TDIF1 and miR-452-5p in gastric cancer tissue were significantly higher than in normal tissue(P<0.05).The expression levels of TDIF1 and miR-452-5p in gastric cancer tissue were positively correlated(r=0.452,P<0.05).Significant differences were found in the expression levels of TDIF1 and miR-452-5p in gastric cancer tissue among patients with different differentiation levels,lymph node metastasis,and TNM staging(P<0.05).Kaplan-Meier survival curve analysis showed that the cumulative survival rates for patients with low expression of TDIF1 and miR-452-5p were 73.33%(33/45)and 75.56%(34/45),respectively,significantly higher than the 48.89%(22/45)and 46.67%(21/45)in those with high expression of both(χ2=5.708,7.993,P=0.017,0.005).Multivariate analysis in-dicated that low tumor differentiation(HR=2.159,95%CI:1.333-3.497),lymph node metastasis(HR=2.637,95%CI:1.479-4.701),TNM stage Ⅲ-IV(HR=2.117,95%CI:1.362-3.290),high expression of TDIF1(HR=2.448,95%CI:1.326-4.521),and high expression of miR-452-5p(HR=3.044,95%CI:1.635-5.666)were independ-ent risk factors for poor prognosis in gastric cancer patients(P<0.05).Conclusion TDIF1 and miR-452-5p are highly ex-pressed in gastric cancer tissue,possibly promoting carcinogenesis.Both are closely correlated with clinical pathological fea-tures and prognosis of gastric cancer.Clinically,TDIF1 and miR-452-5p can be used as potential molecular markers for assess-ing gastric cancer progression and prognosis,providing a reference for personalized treatment strategies.关键词
胃癌/微小RNA-452-5p/末端脱氧核苷酸转移酶相互作用因子1/临床病理特征/预后Key words
Gastric cancer/microRNA-452-5p/TdT-interacting factor 1/Clinical pathological features/Prognosis分类
医药卫生引用本文复制引用
Zhu Jiao,Ren Xiaoyang,Wang Qing,Zhu Min,Gao Nan,Liu Ningning,Luo Miaosha..胃癌组织中TDIF1、miR-452-5p表达水平与临床病理特征及预后的关系[J].保健医学研究与实践,2025,22(10):21-27,7.基金项目
陕西省自然科学基础研究计划项目(2024JC-YBQN-0938). （2024JC-YBQN-0938）
