果树资源学报2026，Vol.7Issue(1)：10-12,65,4.
不同氮源浓度配比对新疆野苹果组培苗生长的影响及机制研究
Effects of Different Nitrogen Source Concentration Ratios on the Growth and Mechanism of Malus sieversii(Xinjiang Wild Apple)Plantlets
摘要
Abstract
[Objective]Tissue rapid propagation experiments of Malus sieversii(Xinjiang wild apple)were conducted to investigate the effects of different nitrogen source concentration ratios on its tissue culture growth,aiming to provide a scientific basis and theoretical reference for the conservation and utilization of Xinjiang wild apple germplasm resources as well as the es-tablishment of a high-efficiency in vitro propagation system.[Methods]Using tissue-cultured seedlings of Xinjiang wild apple as experimental material,five treatment groups were established:T1(100%NO-3),T2(75%NO-3+25%NH4+),T3(50%NO-3+50%NH4+),T4(25%NO-3+75%NH4+),and T5(100%NH4+).A systematic investigation was con-ducted to explore the effects of the concentration ratio of nitrate nitrogen(NO-3)to ammonium nitrogen(NH4+)in 1/2 MS medium on in vitro growth,root development,and physiological characteristics.[Results]The nitrogen source ratio signifi-cantly regulated the morphogenesis of the tissue-cultured seedlings.The T4 treatment demonstrated optimal performance in plant height(20.01 cm),root length(9.45 cm),root number(12),leaf number(22),and leaf thickness(0.11 mm).[Conclusion]These findings provide an optimized nitrogen nutrition strategy for the in vitro conservation and efficient rapid propagation of Malus sieversii germplasm resources.关键词
新疆野苹果/氮源配比/组培苗/根系发育Key words
Malus sieversii(Xinjiang wild apple)/nitrogen source ratio/plantlets/root development分类
农业科技引用本文复制引用
YANG Junxia,SU Xiaolin,MA Jianfang,WU Ye,WANG Ying..不同氮源浓度配比对新疆野苹果组培苗生长的影响及机制研究[J].果树资源学报,2026,7(1):10-12,65,4.基金项目
甘肃省科技厅技术创新引导计划乡村振兴专项(24CXNE005) （24CXNE005）
