海南医科大学学报2025，Vol.31Issue(24)：1841-1850,10.DOI:10.13210/j.cnki.jhmu.20250208.006
基于网络药理学和实验验证探究牛耳枫-辣蓼抗胃黏膜损伤作用及机制
Exploring the protective effects and mechanisms of Daphniphyllum calycinum-Polygonum hydropiper on gastric mucosal injury based on network pharmacology and experimental verification
摘要
Abstract
Objective:To explore the protective effects and mechanisms of Daphniphyllum calycinum-Polygonum hydropiper(DCPH)on gastric mucosal injury.Methods:The active components and main targets of DCPH in the intervention of gastric mu-cosal injury were obtained from network pharmacology,PPI network was constructed,and GO and KEGG enrichment analysis were carried out to screen possible biological processes and signaling pathways.The model of gastric mucosal injury in vitro was established by stimulating GES-1 cells with ethanol,and the protective effects and mechanism of DCPH on GES-1 cells were veri-fied by detecting cell viability,reactive oxygen species(ROS)content,TNF-α and IL-1β expression,mitochondrial membrane potential level,and TLR4 and NF-κB protein expressions.Results:Through network pharmacology,44 potential active compo-nents of DCPH in the intervention of gastric ulcer/gastritis were screened out,including 29 core targets such as TNF,BCL2,EG-FR and TLR4,which involved many biological processes and signaling pathways,such as PI3K-Akt,HIF-1,NF-κB and ROS.The in vitro experimental results showed that compared to the normal group,ethanol could significantly inhibit the activity of GES-1 cells(P<0.001),increase the ROS content(P<0.001),induce the expression of inflammatory factors TNF-α and IL-1β mRNA(P<0.001),significantly reduce the intracellular mitochondrial membrane potential,and significantly up-regulate TLR4 and NF-κB protein expressions.Compared to the model group,DCPH significantly increased the vitality of GES-1 cells induced by ethanol(P<0.05),improved the cell morphology,decreased the intracellular ROS level(P<0.05),inhibited the expressions of TNF-α and IL-1β(P<0.05),restored the mitochondrial membrane potential level,and significantly inhibited the expressions of TLR4 and NF-κB proteins(P<0.05).Conclusion:DCPH alleviates the damage of GES-1 cells and plays an anti-gastric muco-sal injury role by down-regulating the expression of TLR4/NF-κB signaling pathway,reducing the ROS content,inhibiting the ex-pression of inflammatory factors,and restoring the level of mitochondrial membrane potential.关键词
牛耳枫-辣蓼/胃黏膜损伤/GES-1细胞/网络药理学/TLR4/NF-κBKey words
Daphniphyllum calycinum-Polygonum hydropiper/Gastric mucosal injury/GES-1/Network pharmacology/TLR4/NF-κB分类
医药卫生引用本文复制引用
ZHI Yunyun,TIAN Ying,LING Yiwen,NIU Haiyan,REN Shouzhong..基于网络药理学和实验验证探究牛耳枫-辣蓼抗胃黏膜损伤作用及机制[J].海南医科大学学报,2025,31(24):1841-1850,10.基金项目
This study was supported by the National Natural Science Foundation of China(82260920,82474456) （82260920,82474456）
Innovation and Entrepreneurship Training Program for College Students(S202411810030) 国家自然科学基金(82260920,82474456) （S202411810030）
大学生创新创业训练计划项目(S202411810030) （S202411810030）
