海南医科大学学报2025，Vol.31Issue(24)：1869-1877,9.DOI:10.13210/j.cnki.jhmu.20250113.002
菟丝子有效部位筛选与抗乳腺癌作用机制研究
Screening effective sites of Cuscuta and anti-breast cancer mechanism
摘要
Abstract
Objective:To investigate the effective breast cancer sites of Cuscuta and study its mechanism of action.Methods:Extraction of Cuscuta with organic solvents of different polarity and the effective parts were screened under the guidance of pharma-cological effects.The effects of different extraction sites of Cuscuta on the proliferation of 5 tumor cell lines were assessed using MTT colorimetry,and the optimal extraction sites and tumor-sensitive strains were identified.The impact of a 95%alcohol ex-tract from Cuscuta on the morphological changes,migration,invasion,and apoptosis of MCF-7 cells was evaluated through invert-ed microscope observation,strip testing,Transwell assay,and Annexin V-FITC/PI double staining.Western blot was employed to analyze the expression levels of PI3K,p-PI3K,AKT,p-AKT,Bax,and Bcl-2 proteins in MCF-7 cells.Results:The prolifer-ation of MCF-7,SGC-7901,HepG-2,Hela and A549 cells was inhibited by all the extracted parts of Cuscuta.Among them,the inhibitory effect of chloroform and 95%alcohol extract from Cuscuta on the proliferation of MCF-7 cell line was better than the oth-er parts.The median inhibitory concentration(IC50)was 14.3 and 738.0 μg/mL,respectively.After treatment with 400,800,1 200 μg/mL of 95%alcohol extract of Cuscuta on MCF-7 cells,compared to the control group,the cells became round and con-stricted.The space between adjacent cells in the cell mass increased and the migration and invasion ability were gradually weak-ened.Additionally,there was an increase in apoptosis rate(P<0.05),in a time-and dose-dependent manner.Western blot results showed that Cuscuta's 95%alcohol extract could down-regulate the expression of p-PI3K,p-AKT,and Bcl-2 proteins in MCF-7 cells while up-regulating Bax protein expression as well as increasing the ratio of Bax/Bcl-2(P<0.05).Conclusion:Different ex-tracts of Cuscuta demonstrated significant inhibitory effects on the proliferation of 5 tumor cell lines.Notably,the chloroform and 95%alcohol extract of Cuscuta exhibited enhanced inhibitory effects on MCF-7 cells.The anti-breast cancer mechanism attributed to the 95%alcohol extract of Cuscuta may be linked to its modulation of the PI3K/AKT pathway,regulation of downstream Bcl-2 and Bax protein expressions,and participation in the mitochondrial apoptosis pathway.关键词
菟丝子/乳腺癌/PI3K/AKT信号通路/细胞凋亡Key words
Cuscuta/Breast cancer/PI3K/AKT signaling pathway/Apoptosis分类
医药卫生引用本文复制引用
SONG Hui,WANG Yuhan,HUANG Xiaoqiao,SUN Xiangming,LANG Lang,SHAN Wencai,TANG Jing,YU Lei,JI Chenfeng..菟丝子有效部位筛选与抗乳腺癌作用机制研究[J].海南医科大学学报,2025,31(24):1869-1877,9.基金项目
This study was supported by the Natural Science Foundation of Heilongjiang Province(LH2024H036) （LH2024H036）
Heilongjiang Province"Double First Class"Discipline Collaborative Innovation Achievement Project(LJGXCG2024-P33) （LJGXCG2024-P33）
Postdoctoral Program of Heilongjiang Province(LBH-Z20023) （LBH-Z20023）
Traditional Chinese Medicine Research Project of Heilongjiang Provincial Administration of Traditional Chinese Medicine(ZHY2024-098) 黑龙江省自然科学基金项目(LH2024H036) （ZHY2024-098）
黑龙江省"双一流"学科协同创新成果项目(LJGXCG2024-P33) （LJGXCG2024-P33）
黑龙江省博士后项目(LBH-Z20023) （LBH-Z20023）
黑龙江省中医药管理局中医药科研课题项目(ZHY2024-098) （ZHY2024-098）
