中医学报2026，Vol.41Issue(1)：38-47,10.DOI:10.16368/j.issn.1674-8999.2026.01.007
基于生物信息学探讨重症哮喘气道重塑差异表达基因及中药预测
Bioinformatics-Based Investigation of Differentially Expressed Genes in Airway Remodeling of Severe Asthma and TCM Application Prediction
摘要
Abstract
Objective:To explore the key genes involved in airway remodeling in severe asthma(SA)based on bioinformatics and to map database-predicted traditional Chinese medicines,providing insights for clinical treatment of SA.Methods:SA-related datasets were ob-tained from the GEO database,and airway remodeling genes were retrieved from the Genecards database.Genes associated with SA airway remodeling were identified,and functional enrichment analysis was performed on these genes.Machine learning methods were then used to further screen for key differentially expressed genes in SA airway remodeling.ROC validation was conducted using both internal and exter-nal GEO datasets.Potential traditional Chinese medicines targeting SA airway remodeling were identified by mapping the key genes with the Coremine database.Results:This study identified a total of 53 differentially expressed genes in severe asthma(SA),including 29 up-regulated genes and 24 downregulated genes.Twelve genes related to airway remodeling in SA were obtained from which six characteristic genes were selected.A total of 120 traditional Chinese medicines(TCMs)were predicted to potentially act on SA airway remodeling,mainly including Heat-clearing drugs,tonifying drugs as well as Phlegm-resolving and cough-relieving bronchodilators.Molecular doc-king showed that the characteristic genes had good binding with the core components of high-frequency TCMs.Conclusion:Vascular en-dothelial growth factor A,fibronectin,CXADR,keratin,interleukin-1 receptor type 2,and interleukin-18 receptor 1 may be potential bi-omarkers for the treatment of airway remodeling in SA.It is speculated that TCMs such as Aidicha(Ardisia herb),Banzhilian(Scutellaria barbata),Nzhenzi(Ligustrum lucidum)and Baiguo(Ginkgo seed)may be potential therapeutic drugs for SA airway remodeling.关键词
重症哮喘/气道重塑/差异表达基因/生物信息学/中药预测/分子对接Key words
severe asthma/airway remodeling/differentially expressed gene/bioinformatics/TCM application prediction/molecular doc-king分类
医药卫生引用本文复制引用
ZHANG Xinxin,PENG Xiaoyun,LIANG Huiqiu,LUO Zhiyan,WEN Wujin,JIANG Yong,LIAO Gang..基于生物信息学探讨重症哮喘气道重塑差异表达基因及中药预测[J].中医学报,2026,41(1):38-47,10.基金项目
广东省自然科学基金面上项目(2024A1515012183) （2024A1515012183）
广东省中医药局课题项目(20241265) （20241265）
广东省重点科室(中西医协同科室)建设项目 （中西医协同科室）
深圳市"医疗卫生三名工程"建设项目(SZZYSM202206013) （SZZYSM202206013）
深圳市宝安区中医药临床研究专项课题项目(2023ZYYLCZX-9,2023ZYYLCZX-11) （2023ZYYLCZX-9,2023ZYYLCZX-11）
深圳市宝安区医疗卫生科研项目(2023JD124,2023JD107,2023JD105,2023JD110,2024JD289,2024JD293,2024JD316) （2023JD124,2023JD107,2023JD105,2023JD110,2024JD289,2024JD293,2024JD316）
深圳市宝安区医学会医疗卫生科研项目(BAYXH2024011) （BAYXH2024011）
深圳市中西医结合医院院内课题项目(YJ-2023-106,YJ-2023-104,YJ-2023-115) （YJ-2023-106,YJ-2023-104,YJ-2023-115）
