湖南生态科学学报2025，Vol.12Issue(4)：99-104,6.DOI:10.3969/j.issn.2095-7300.2025.04.013
黑老虎花瓣内生真菌的高效显微检测方法研究
Efficient Microscopic Detection of Endophytic Fungi in Kadsura coccinea Petals
摘要
Abstract
[Objective]To tackle sectioning challenges and background noise encountered in traditional detection of endophytic fungi in Kadsura coccinea(a plant characterized by highly lignified and deeply pigmented tissues),this study aims to develop a rapid and efficient aniline blue staining-micro-scopic detection method for endophytic fungi based on petal tissue.[Method]Petals of K.coccinea were used as experimental materials.Samples were prepared via hand-tearing and whole-mount methods,stained with varying concentrations of aniline blue solution,and their detection effects on endophytic fungi were systematically observed and compared.[Result]Endophytic fungi were successfully detected in K.coccinea petals,confirming the feasibility of using petals as detection materials.Notably,samples from the hand-tearing method exhibited clearer microscopic fields,better hyphal staining,and significantly higher contrast between hyphae and petal background than the whole-mount method.[Con-clusion]This study established an in situ detection technique for endophytic fungi in K.coccinea petals,verifying that the hand-tearing method yields superior observation results to the whole-mount method.With advantages of rapidity,clarity,and low cost,this technique provides reliable technical support for microecological research on K.coccinea and other medicinal plants with similar morphological characteris-tics.关键词
黑老虎/花瓣/内生真菌/组织化学染色/苯胺蓝染色/原位检测Key words
Kadsura coccinea/petal/endophytic fungi/histochemical staining/aniline blue staining/in situ detection分类
生物科学引用本文复制引用
ZHU Minjie,ZHOU Angang,DENG Ye,HU Chunmei..黑老虎花瓣内生真菌的高效显微检测方法研究[J].湖南生态科学学报,2025,12(4):99-104,6.基金项目
湖南环境生物职业技术学院院长科研基金资助(项目编号:NY2022-04,GC2025-06) （项目编号:NY2022-04,GC2025-06）
