华北农学报2025，Vol.40Issue(6)：11-20,10.DOI:10.7668/hbnxb.20195302
低温胁迫条件下大豆萌发期转录组分析
Transcriptome Analysis of Germinating Soybeans under Low Temperature Stress
摘要
Abstract
The soybean germination stage is greatly affected by low-temperature stress,which can have a signifi-cant impact on yield.In order to explore genes related to the response of soybean germination to low-temperature stress and investigate the biological processes underlying soybean germination tolerance to cold,this study conducted tran-scriptome sequencing on seeds germinating for three days from eight materials showing significant differences in low-temperature tolerance during germination.Differential expressed genes(DEGs)between materials tolerant and sen-sitive to low temperatures were identified and subjected to GO enrichment analysis,KEGG pathway enrichment anal-ysis,and transcription factor analysis.Among 231 DEGs identified in 15 contrasting groups of low-temperature toler-ance,159 DEGs were up-regulated and 72 DEGs were down-regulated in cold-sensitive soybeans.GO enrichment a-nalysis revealed that DEGs were mainly involved in biological processes such as cellular processes(GO:0009987),metabolic processes(GO:0008152),biological regulation(GO:0065007),response to stimulus(GO:0050896),binding(GO:0005488),transporter activity(GO:0005215),and transcription regulator activity(GO:0140110).KEGG pathway enrichment analysis indicated that DEGs were significantly enriched in starch and sucrose metabo-lism pathways(ko00500).Genes involved in seed development(Glyma.03G144400,Glyma.19G147200,Glyma.10G027600,Glyma.10G247500,Glyma.20G147600),metabolic reactions(Glyma.05G004300,Glyma.17G086400),and genes encoding glutathione oxidase(Glyma.01G219400)were up-regulated in cold-sensitive materials.Fifteen transcription factors from families such as MYB,AP2/ERF,and NAC were identified among the 231 differentially expressed genes,suggesting that soybeans respond to low-temperature stress during germination by regulating various biological processes,metabolic pathways,and signal transduction pathways.关键词
大豆/萌发期/低温胁迫/转录组/候选基因Key words
Soybean/Germination stage/Low temperature stress/Transcriptome/Candidate gene分类
农业科技引用本文复制引用
CHEN Yuehan,WEI Yu,FENG Yan,ZHAO Li,YAN Long,YANG Qing,LIU Zhi..低温胁迫条件下大豆萌发期转录组分析[J].华北农学报,2025,40(6):11-20,10.基金项目
河北省科学技术厅科技业务项目 （）
河北省农林科学院科技创新人才队伍建设项目(C24R0308,C24R0302) （C24R0308,C24R0302）
