华北农学报2025，Vol.40Issue(6)：21-30,10.DOI:10.7668/hbnxb.20195788
甘蓝型油菜Metacaspase家族基因鉴定及其对干旱胁迫响应分析
Identification of Metacaspase Family Genes in Brassica napus and Analysis of Gene Expression under Drought Stress
摘要
Abstract
Metacaspase(MC)belongs to arginine/threonine specific protease,studies have shown that it plays a role in programmed cell death.To investigate the distribution of MC family genes in the genome of Brassica napus and whether they respond to drought stress,this study systematically analyzed the physicochemical properties,phy-logeny,gene structure,conserved domains,cis-acting elements,and expression patterns of MC family genes under drought(PEG6000)and abscisic acid(AB A)stress in B.napus.A total of 25 BnMC genes were identified.Chromo-somal localization showed that the 25 BnMCs were distributed on 13 chromosomes.Subcellular localization predic-tion showed that 17 members of the BnMC family were localized in the nucleus and seven members were in the cyto-plasm.The phylogenetic tree classified BnMC into two major classes(Type Ⅰ and Type Ⅱ)and four branches(Group A,Group B,Group C,and Group D).BnMCs of the same branch had similar gene structure and conserved motif distribution.The core promoter regions of BnMC contained four types of cis-acting elements:light response ele-ment,phytohormone response element,plant growth and development response element and stress response element.Among all the cis-acting elements related to abiotic stress responses,the abscisic acid response element(ABRE)was the most abundant,with a total of 79.All members contained this cis-acting element.The transcriptome sequen-cing revealed that the expressions of BnMC10,BnMC22,BnMC1,BnMC12 and BnMC8 were up-regulated and the expressions of BnMC4 and BnMC5 were down-regulated after drought treatment.The qRT-PCR assay showed Bn-MC10,BnMC8,BnMC1 and BnMC12 genes were expressed in both roots and leaves and were up-regulated by both PEG6000 and ABA,with BnMC1 showing the most significant up-regulating changes.In summary,the response of B.napus to drought stress involves the regulation of the expression level of MC family genes.关键词
甘蓝型油菜/Metacaspase/基因家族/干旱胁迫Key words
Brassica napus/Metacaspase/Gene family/Drought stress分类
农业科技引用本文复制引用
LI Tian,WANG Daojie,ZHANG Xiaojuan,HOU Yangzi..甘蓝型油菜Metacaspase家族基因鉴定及其对干旱胁迫响应分析[J].华北农学报,2025,40(6):21-30,10.基金项目
陕西理工大学人才启动项目(SLGRCQD2115) （SLGRCQD2115）
陕西省科技厅项目(2023-YBNY-070) （2023-YBNY-070）
三秦英才,陕西省级一流团队"陕西理工大学污染土壤修复与资源利用创新团队"项目 （）
