华北农学报2025，Vol.40Issue(6)：31-39,9.DOI:10.7668/hbnxb.20195949
花生荚果响应干旱和渍涝胁迫的转录组学分析
Transcriptomic Analysis of Peanut Pods in Response to Drought and Waterlogging Stresses
摘要
Abstract
To elucidate the molecular mechanisms underlying peanut pod responses to water stress,this study employed pot experiments combined with transcriptomic analysis.Using well-watered conditions as the control,we systematically investigated the effects of periodic drought and waterlogging stress during the flowering-pegging stage on yield,quality,and gene expression in peanut pods.Results demonstrated that both drought and waterlogging stresses significantly reduced peanut pod yield(by 26.43％and 77.69％,respectively)and crude fat content in kernels(by 9.46,6.71 percentage points,respectively).Transcriptomic analysis further revealed 1 525 and 1 382 differentially expressed genes(DEGs)in the drought-stress and waterlogging-stress groups compared to the control,respectively,with down-regulated expression being predominant in both sets of DEGs.Specifically,drought stress suppressed six key metabolic processes related to lipid and fatty acid metabolism in peanut pods,with 88.38％of associated genes showing downregulated expression,indicating that lipid metabolic disruption may be the primary cause of yield and quality reduction under drought.Waterlogging stress predominantly interfered with pod metabo-lism and defense functions by downregulating genes associated with catalytic activity,transmembrane transport,redox reactions,and biosynthesis of secondary metabolites.Moreover,KEGG enrichment analysis indicated that metabolic pathways and biosynthesis of secondary metabolites were significantly affected under both water stress conditions.Key gene validation via qRT-PCR corroborated the RNA-seq data,confirming the reliability of the transcriptomic findings.In summary,this research elucidates the molecular basis of peanut pod response to water stress at the transcriptome level,demonstrating that lipid metabolic disruption is the primary factor underlying yield reduction and quality deterioration under drought stress,whereas peanut pods mitigate the adverse effects of waterlogging mainly by modulating the expression of genes associated with redox homeostasis and metabolic pathways.关键词
花生荚果/干旱胁迫/渍涝胁迫/转录组/籽仁品质/产量Key words
Peanut pod/Drought stress/Waterlogging stress/Transcriptome/Kernel quality/Yield分类
农业科技引用本文复制引用
YU Tianyi,FAN Zhaobo,ZHANG Jialei,LU Ya,WU Juxiang,YANG Jishun,LI Shangxia,WU Zhengfeng,WAN Shubo..花生荚果响应干旱和渍涝胁迫的转录组学分析[J].华北农学报,2025,40(6):31-39,9.基金项目
青岛市自然科学基金原创探索项目(24-4-4-zrjj-146-jch) （24-4-4-zrjj-146-jch）
国家重点研发计划课题(2021YFB3901303) （2021YFB3901303）
山东省农业科学院农业科技创新工程项目(CXGC2025G13,CXGC2025A08) （CXGC2025G13,CXGC2025A08）
山东省重点研发计划项目(2021LZGC026) （2021LZGC026）
青岛市科技惠民计划项目(25-1-5-xdny-21-nsh) （25-1-5-xdny-21-nsh）
