华北农学报2025，Vol.40Issue(6)：40-49,10.DOI:10.7668/hbnxb.20195964
基于转录组和代谢组联合分析裸大麦苗期耐盐分子机制
Molecular Mechanisms of Salt Tolerance in Naked Barley at Seedling Stage Based on Integrated Transcriptomic and Metabolomic Analysis
摘要
Abstract
Salt stress causes a significant threat to crop yield and quality.As one of the pioneer crop species in salt tolerance research,barley holds critical significance;the exploration of its salt tolerance mechanisms is capable of providing a theoretical foundation for crop salt-tolerance breeding programs.Two naked barley landraces,namely B87 with salt-sensitivity and B94 with salt-tolerance,were employed as experimental materials.At the three-leaf stage,their seedlings were exposed to a 200 mmol/L NaCl treatment for 7 days.Subsequent to the treatment,the a-bove-ground tissues were collected for transcriptomic and metabolomic sequencing.By means of integrated multi-omics analysis,this study was designed to elucidate the molecular mechanisms governing salt tolerance in naked bar-ley.The results demonstrated that 2 240 differentially expressed genes(DEGs)and 198 differentially abundant me-tabolites(DAMs)were identified in B87 via transcriptomic and metabolomic profiling,whereas 923 DEGs and 232 DAMs were detected in B94.Venn diagram analysis further revealed that the salt-tolerant naked barley B94 con-tained 480 specific DEGs and 129 specific DAMs.Furthermore,GO and KEGG analyses were separately performed on the DEGs and DAMs.And the DEGs of B94 were significantly enriched in 11 unique pathways,while its DAMs were only significantly enriched in 1 unique pathway.In addition,correlation analysis between the transcriptome and metabolome was conducted,and it was found that the changes in genes and metabolites exhibited both consis-tency and inconsistency.These research efforts not only enhance the current understanding of the molecular mecha-nisms underlying salt tolerance in naked barley,but also provide valuable insights and candidate targets for the de-velopment of salt-tolerant naked barley cultivars in future breeding.关键词
裸大麦/耐盐性/转录组/代谢组/定量PCRKey words
Naked barley/Salt tolerance/Transcriptome/Metabolome/qPCR分类
农业科技引用本文复制引用
LIU Shisen,CHEN Zhiwei,YANG Yicheng,FENG Shiji,GUO Zhenzhu,ZHANG Shuwei,GUO Guimei,WANG Yu,ZHOU Longhua,LIU Chenghong..基于转录组和代谢组联合分析裸大麦苗期耐盐分子机制[J].华北农学报,2025,40(6):40-49,10.基金项目
农业农村部国家大麦青稞产业技术体系(CARS-05-01A-02) （CARS-05-01A-02）
西藏自治区科技厅西藏自治区科技计划项目(XZ202501ZY0029) （XZ202501ZY0029）
上海市农业农村委上海市科技兴农项目(2019-02-08-00-08-F01109) （2019-02-08-00-08-F01109）
上海市农科院卓越团队建设项目(沪农科卓(2022)018) （沪农科卓(2022）
