华北农学报2025，Vol.40Issue(6)：50-61,12.DOI:10.7668/hbnxb.20195651
玉米BAM基因家族鉴定及响应雹灾胁迫的表达分析
Identification of BAM Gene Family in Maize and Analysis of Expression in Response to Hail Stress
摘要
Abstract
β-Amylase(BAM)is involved in regulating various biological processes in plants and responds to multiple external stimuli such as hormones and abiotic stress.To explore the characteristics and expression patterns of the BAM gene family in maize,we performed a genome-wide identification of the maize BAM gene family using bioin-formatics methods.We also analyzed the encoded proteins,chromosomal localization,gene structure,cis-acting ele-ments,synteny analysis,tissue-specific expression,and the expression patterns of the genes after simulated hail stress and melatonin treatment.The results showed that 16 ZmBAM members were identified in maize,all of which contained the typical Glyco_hydro_14 domain,with most of them being hydrophilic proteins.Phylogenetic analysis in-dicated that ZmBAM protein can be divided into three groups,with genes in the same group sharing similar gene structures and motif distributions.Cis-acting element analysis suggested that the expression of most ZmBAM genes might be related to plant hormones,abiotic stress,and light responses.Synteny analysis revealed a certain level of homology between the maize BAM gene family and those of Arabidopsis and soybean,with a closer relationship to rice.qRT-PCR analysis showed that,under simulated hail stress,most genes were upregulated,and their expression was significantly upregulated after melatonin treatment.This study provided a reference for further understanding the function of the maize BAM gene family in response to abiotic stress.关键词
玉米/BAM/基因家族/生物信息学Key words
Maize/BAM/Gene family/Bioinformatics分类
农业科技引用本文复制引用
JIANG Aonan,CHEN Xushuang,YANG Qifan,LIU Guangzhou,CUI Yanhong,BIAN Dahong..玉米BAM基因家族鉴定及响应雹灾胁迫的表达分析[J].华北农学报,2025,40(6):50-61,12.基金项目
"十四五"国家重点研发计划项目(2023YFD2301502) （2023YFD2301502）
河北省玉米产业技术体系(HBCT2023020101) （HBCT2023020101）
