华北农学报2025，Vol.40Issue(6)：62-70,9.DOI:10.7668/hbnxb.20195920
棉花GhCRK26基因克隆、亚细胞定位及表达分析
Cloning,Subcellular Localization and Expression Analysis of GhCRK26 Gene in Cotton
摘要
Abstract
As an important subfamily of receptor kinases,cysteine-rich receptor kinases play a key role in plant growth and development.The aim of this study was to analyze the relationship between the GhCRK26 gene and the devel-opment of cotton fiber,and to provide a theoretical basis for the improvement of cotton fiber quality.This study selected upland cotton Line 9 and sea island cotton Xinhai 16,and collected samples at different periods of fiber development and root,stem and leaf tissues.The expression pattern of GhCRK26 was analyzed by qRT-PCR;the gene was cloned by PCR;a phylogenetic tree was constructed with the help of bioinformatics tools to predict the physicochemical properties of the protein,its transmembrane structure and signal peptide;the promoter cis-acting elements were analyzed by the PlantCare database;and the localization of the protein was clarified by the subcellular localization assay.The results showed that the expression of GhCRK26 gene showed highly significant differences at 10,20 and 30 days after flowering in two varieties;the highest expression levels were found in the leaves of Line 9,while in Xinhai 16,the expression of stem and leaves did not show significant differences.A 2 049 bp CDS sequence encoding 682 amino acids was successfully cloned.The evolu-tionary tree showed that GhCRK26 protein was the most distantly related to Hibiscus trionum,and the closest to Gossypi-um tomentosum and Gossypium barbadense;the protein was hydrophilic and unstable,containing a transmembrane structure and a signal peptide;methyl jasmonate and abscisic acid response elements were identified in the promoter region;and the subcellular localization confirmed that it was localized in the cell membrane.The above studies pro-vide a basis for further in-depth analysis of the function of GhCRK26 gene in fiber development.关键词
棉花/纤维/GhCRK26/基因克隆/表达分析/亚细胞定位Key words
Cotton/Fiber/GhCRK26/Gene cloning/Expression analysis/Subcellular localization分类
农业科技引用本文复制引用
LI Jing,SONG Wu,LI Shengmei,GAO Wenwei,PANG Bo,ZHANG Ru,ZHAERJIAMALI Abudubieke,WANG Zhengrui,SHI Shunyu,FAN Zhenzhen,MA Jingjing,CHEN Jialin..棉花GhCRK26基因克隆、亚细胞定位及表达分析[J].华北农学报,2025,40(6):62-70,9.基金项目
国家重点研发计划子课题(2021YFD1900802-4) （2021YFD1900802-4）
新疆维吾尔自治区"天池英才"引进计划项目 （）
新疆维吾尔自治区自然科学基金项目(2025D011396) （2025D011396）
2024年度新疆农业职业技术大学资助课题(XJNZYBS202401) （XJNZYBS202401）
