华北农学报2025，Vol.40Issue(6)：71-76,6.DOI:10.7668/hbnxb.20195990
马铃薯核运输蛋白IMPα2基因克隆及功能分析
Gene Cloning and Functional Research of Importin IMPα2 in Solanum tuberosum
摘要
Abstract
To investigate the function of StIMPα in potato,this study used the potato cultivar Kexing No.1 as material and successfully cloned the StIMPα2 gene via PCR.Bioinformatic analysis revealed that the coding se-quence(CDS)of StIMPα2 had a length of 1 590 bp,encoding a protein containing the typical domains of the IMPα family.Phylogenetic tree analysis indicated that StIMPα2 was most closely related to AtIMPα-1 and AtIMPα-2 from Arabidopsis thaliana,suggesting functional conservation.Furthermore,analysis of the StIMPα2 promoter re-gion identified multiple cis-acting elements associated with responses to biotic and abiotic stresses.To determine its subcellular localization,a StIMPα2-GFP fusion expression vector was constructed and transiently expressed in leav-es of Nicotiana benthamiana via Agrobacterium-mediated transformation.Confocal laser scanning microscopy showed that the GFP fluorescence signal was specifically enriched in the nucleus,confirming that StIMPα2 is a nuclear-lo-calized protein.Expression pattern analysis demonstrated that StIMPα2 expression was significantly induced by abi-otic stresses such as low temperature,high salinity,and drought,as well as by BTH(benzothiadiazole).For func-tional validation,StIMPα2 was overexpressed in N.benthamiana via Agrobacterium infiltration,followed by inocu-lation with Phytophthora infestans.Pathological phenotype analysis showed that compared with the control,the le-sion area on leaves overexpressing StIMPα2 was significantly reduced.Meanwhile,Quantitative Real-time PCR de-tection of P.infestans biomass confirmed a significant decrease in pathogen biomass in StIMPα2-overexpressing plants.In conclusion,these results indicate that StIMPα2 is a nuclear-localized protein induced by various biotic and abiotic stresses,and it enhances resistance to P.infestans by positively regulating plant immune responses.关键词
马铃薯/StIMPα2/克隆/致病疫霉/抗性Key words
Solanum tuberosum/StIMPα2/Cloning/Phytophthora infestans/Resistance分类
农业科技引用本文复制引用
ZHANG Yifan,LIN Rui,ZHOU Na,HU Anqi,BAI Wei..马铃薯核运输蛋白IMPα2基因克隆及功能分析[J].华北农学报,2025,40(6):71-76,6.基金项目
国家自然科学基金项目(32060613) （32060613）
