华北农学报2025，Vol.40Issue(6)：77-84,8.DOI:10.7668/hbnxb.20195979
枣ZjIAA9生物信息学分析及功能验证
Bioinformatics Analysis and Functional Verification of ZjIAA9 in Ziziphus jujuba Mill
摘要
Abstract
The members of the Aux/IAA gene family encode proteins that regulate various developmental proces-ses in plants,such as embryogenesis and seed development,by modulating the auxin(IAA)signal transduction path-way.To explore the role of Aux/IAA family genes in jujube embryo development,this study performed bioinformatics analysis and functional verification on the key genes of the Aux/IAA family that affect jujube embryo development,u-sing transcriptome data of Huizao(a jujube variety with high embryo fertility)and Kongfusucui(a jujube variety with low embryo fertility).Results showed that an Aux/IAA family gene sequence with a length of 1 731 bp was obtained via gene cloning,which encoded 362 amino acids.Protein structure prediction indicated that its secondary structure was mainly composed of random coil and that it had a typical conserved domain of the IAA9 protein;thus,it was named ZjIAA9.Homology analysis revealed that ZjIAA9 was closely related to RGQ29_009000 in Quercus rubra and CFP56_014209 in Quercus suber.Transgenic Micro-Tom tomato plants were obtained via Agrobacterium-mediated leaf disc transformation.Determination of auxin content in transgenic Micro-Tom tomato plants showed that the IAA content in the pulp of transgenic plants was higher than that in non-transgenic plants,indicating that ZjIAA9 may regulate auxin biosynthesis.Compared with wild-type plants,transgenic plants showed a seedless or few-seeded phe-notype,suggesting that jujube ZjIAA9 may be involved in regulating jujube embryo development.关键词
枣/胚发育/ZjIAA9/生物信息学分析/功能验证Key words
Jujube/Embryo development/ZjIAA9/Bioinformatics analysis/Functional verification分类
农业科技引用本文复制引用
YANG Mengfan,LI Yi,MA Qing,REN Haiyan,WANG Yongkang,ZHAO Ailing,XUE Xiaofang,SU Wanlong,SHI Meijuan,LIU Li..枣ZjIAA9生物信息学分析及功能验证[J].华北农学报,2025,40(6):77-84,8.基金项目
山西省科技重大专项计划"揭榜挂帅"项目(202201140601027) （202201140601027）
山西省自然科学基金项目(202103021224147) （202103021224147）
农业农村部种质资源保护专项(111721301354052002) （111721301354052002）
国家科技资源共享服务平台项目(NHGRC2023-NH12-1) （NHGRC2023-NH12-1）
现代农业产业技术体系专项(CARS-30-1-07,CARS-30-ZZ-22) （CARS-30-1-07,CARS-30-ZZ-22）
