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绣球HmMYB73生物信息学分析及其耐铝功能验证

CHEN Shuangshuang LIU Yiming CHEN Wenjuan FENG Jing CHEN Huijie LIU Xintong DU Xiaohua DENG Yanming

华北农学报2025，Vol.40Issue(6)：85-91,7.

华北农学报2025，Vol.40Issue(6)：85-91,7.DOI:10.7668/hbnxb.20195913

绣球HmMYB73生物信息学分析及其耐铝功能验证

Bioinformatics Analysis and Functional Validation of Aluminum Tolerance of HmMYB73 in Hydrangea macrophylla

CHEN Shuangshuang ¹LIU Yiming ²CHEN Wenjuan ³FENG Jing ¹CHEN Huijie ¹LIU Xintong ¹DU Xiaohua ⁴DENG Yanming⁵

作者信息

1. Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
2. Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China||School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,China
3. Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China||Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210014,China
4. School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,China
5. Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China||School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,China||Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210014,China
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摘要

Abstract

Hydrangea macrophylla is an ornamental plant with high aluminum(Al)tolerance and strong Al accu-mulation capacity.Its flower color is easily influenced by soil pH and the Al3+content in sepals,yet the mechanisms un-derlying its Al tolerance remain poorly understood.The MYB transcription factor family plays a crucial role in plant stress responses.To investigate the function of MYB transcription factors in Al tolerance in H.macrophylla,this study used H.macrophylla'Endless Summer'as experimental material.Gene cloning,bioinformatics,co-expression network,expression pattern analysis and yeast functional validation of HmMYB73 were conducted.The results showed that HmMYB73 encoded 266 amino acids,contained a typical R2R3 domain,and belonged to the R2R3-MYB subfamily.Genes co-expressed with HmMYB73 were involved in biological functions such as catalytic activity,transporter activity,response to stimulus and detoxification.Under Al treatment,HmMYB73 was significantly upregulated in the roots,leaves,and sepals of H.macro-phylla,with the highest expression level observed in the roots,which was 16 times that of the control.Furthermore,overex-pression of HmMYB73 significantly enhanced yeast tolerance to aluminum stress.In summary,HmMYB73 may be involved in the biological regulation during aluminum stress in H.macrophylla.

关键词

绣球/MYB73/耐铝性/共表达网络/酵母功能验证

Key words

Hydrangea macrophylla/MYB73/Aluminum tolerance/Co-expression network/Yeast functional validation

分类

农业科技

引用本文复制引用

CHEN Shuangshuang,LIU Yiming,CHEN Wenjuan,FENG Jing,CHEN Huijie,LIU Xintong,DU Xiaohua,DENG Yanming..绣球HmMYB73生物信息学分析及其耐铝功能验证[J].华北农学报,2025,40(6):85-91,7.

基金项目

国家自然科学基金项目(32371956) （32371956）

江苏省农业科技自主创新资金项目(CX(22)2035) （CX(22）

江苏省种业振兴"揭榜挂帅"项目(JBGS[2021]097) （JBGS[2021]097）

中央财政林业科技推广示范项目(苏(2024 TG)03) （苏(2024 TG）

华北农学报

OA北大核心

ISSN：1000-7091

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