华北农学报2025，Vol.40Issue(6)：92-99,8.DOI:10.7668/hbnxb.20195829
钩藤开花相关基因UrAP1的克隆及表达分析
Cloning and Expression Analysis of the Flowering Related Gene UrAP1 in Uncaria rhynchophylla
摘要
Abstract
During the reproductive phase of Uncaria rhynchophylla,stem hooks undergo conversion into pedun-cles,consequently diminishing stem hook yield.To identify key regulatory factors governing this organ homology shift between stem hooks and peduncles in U.rhynchophylla,tissues from stem hooks and flowers were utilized to isolate its floral meristem gene,UrAP1(APETALA1).Comprehensive bioinformatic characterization was subsequently per-formed to elucidate the physicochemical properties of its encoded product.Concurrently,qRT-PCR analysis was con-ducted to quantifiy the relative transcript abundance of UrAP1 across distinct tissues of vegetative and reproductive branches,aiming to clarify its functional role and regulatory network in floral organogenesis.Results demonstrated that the UrAP1 coding sequence(CDS)spanned 729 bp,encoding a 242 amino acid polypeptide.This sequence ex-hibited substantial homology(94％identity)with the AP1 gene from Cephalanthus occidentalis and harbored conserved MADS-box and K-box domains.Furthermore,the UrAP1 protein lacked transmembrane domains,with primary localization predicted within the nucleus.UrAP1 expression was detectable and relatively stable across leaves,stems,stem nodes,and stem hooks of vegetative shoots.In contrast,its transcript levels varied significantly in reproductive shoots,showing dis-tinct abundances in leaves,stems,stem nodes,peduncles,and flower buds.Expression peaked in young buds during early flowering stages and subsequently declined as buds matured.Comparison of the expression profiling between the two growth phases suggests a crucial role of UrAP1 in regulating floral bud differentiation in U.rhynchophylla.关键词
钩藤/UrAP1基因/生物信息学/基因表达/克隆Key words
Uncaria rhynchophylla/UrAP1 gene/Bioinformatics/Gene expression/Cloning分类
农业科技引用本文复制引用
LI Yuqiong,FU Jine,LI Linxuan,WAN Lingyun,QIN Ya,HUANG Qiulan,SHEN Jie,YANG Cuihong,LIANG Wenjing,WEI Shugen,PAN Limei,GUI Lingjian..钩藤开花相关基因UrAP1的克隆及表达分析[J].华北农学报,2025,40(6):92-99,8.基金项目
国家自然科学基金项目(82360753,81903752) （82360753,81903752）
广西自然科学基金项目(2019GXNSFBA185026) （2019GXNSFBA185026）
广西药用植物园重点研发项目(桂药重202401) （桂药重202401）
广西特色药材关键技术研究推广项目(GZKJ2314) （GZKJ2314）
广西药用植物园科研基金项目(桂药基202013) （桂药基202013）
广西中药材品质创新研究团队项目(GZKJ2305) （GZKJ2305）
