湖南生态科学学报2025，Vol.12Issue(4)：12-22,11.DOI:10.3969/j.issn.2095-7300.2025.04.002
Bt蛋白对铜铅复合污染土壤重金属形态及细菌群落的影响
Effects of Bt Protein on Cu and Pb Morphology and Bacterial Community in Soil Contaminated by Heavy Metals
摘要
Abstract
[Objective]The effects of Bt protein on copper and lead speciation and bacterial commu-nities in soil were investigated so as to provide a theoretical basis for assessing the potential ecological risks of its introduction into heavy metal-contaminated soil ecosystems.[Method]Using a typical Bt in in-secticidal protein—Cry1Ac protein and soil heavy metals(Cu,Pb)as subjects,through short-term(1 day)and long-term(60 days)soil incubation experiments,soil physicochemical properties,Cu/Pb spe-ciation distribution,and bacterial communities were measured.[Result]Results indicate that increased Bt protein content elevates available Cu and Pb concentrations in soil.Correlation analysis reveals a high-ly significant positive correlation between soil acid-extractable Cu and Bt protein initial concentration a-nalysis levels,while the reducible fraction of Cu shows a significant negative correlation with both param-eters.Short-term(1 day)Bt protein exposure slightly reduces the bacterial richness index.Compared to 1-day cultures,60-day cultures exhibited significantly decreased bacterial community richness and diver-sity,primarily attributed to sustained heavy metal stress.[Conclusion]The Bt protein released from Bt crops,when entering soil contaminated with heavy metals,can enhance the bioavailability of Cu and Pb through direct complexation or competitive adsorption of sites.This process temporarily suppresses bacte-rial community richness.Over time,however,it synergizes with heavy metal stress to alter the structure of bacterial communities.Future studies should investigate the long-term interactions between these ele-ments under field conditions to provide a more comprehensive theoretical framework for ecological risk as-sessment of transgenic crop cultivation in heavy metal-contaminated farmland.关键词
Bt蛋白/土壤理化性质/重金属形态/细菌多样性/铜铅污染Key words
Bt protein/soil physical and chemical properties/heavy metal morphology/bacterial di-versity/Cu and Pb pollution分类
生物科学引用本文复制引用
WU Daoxiong,ZENG Hong,YANG Sipei,QIN Pufeng,LIANG Yunshan..Bt蛋白对铜铅复合污染土壤重金属形态及细菌群落的影响[J].湖南生态科学学报,2025,12(4):12-22,11.基金项目
国家自然科学基金项目(41877491) （41877491）
湖南省自然科学基金项目(2022JJ30307)和湖南农业大学"1515"人才项目 （2022JJ30307）
