湖南生态科学学报2025，Vol.12Issue(4)：29-35,7.DOI:10.3969/j.issn.2095-7300.2025.04.004
麝亚百合与6个亚洲百合原种及栽培品种杂交亲和性及杂种后代增殖技术研究
Study on the Hybridization Compatibility and Proliferation Techniques of Hybrid Offspring between LA Lily and Six Original and Cultivated Varieties of Asian Lilies
摘要
Abstract
[Objective]This study aims to explore the hybrid affinity between three LA lilies and six native and cultivated varieties of Asian lilies,with the expectation of breeding LA lily hybrid offspring with hybrid vigor.[Method]Three varieties of LA lily were subjected to distant hybridization with six o-riginal and cultivated varieties of lily from Asia.After hybridization,the fruit expansion rate and embryo formation rate of each hybrid combination were statistically analyzed.The embryo seeds were cultured in vitro with ovules to obtain hybrid seedlings,and then different NAA(naphthyl acetic acid)were set.The concentration of NAA was used to screen out the best proliferation medium formula suitable for the prolif-eration in hybrid offspring.[Result]Among the hybrid combinations with three LA lilies as the female parent,only'Benevento'and six lilies as the male parent all obtained enlarged fruits,and the fruit en-largement rate was all greater than 80%.The hybridization compatibility between the three LA lilies and Lilium leichtlinii var.maximowiczii was the highest.After hybridization,the fruit expansion rate and the average embryo rate were both higher than those of other hybridization combinations,and all of them ob-tained hybrid offspring.The optimal proliferation medium formula for the adventitious buds of the hybrid offspring of'Benevento'×Lilium leichtlinii var.maximowiczii is MS+1 mg/L 6-BA+0.05 mg/L NAA+30 g/L sucrose+7 g/L agar,with a pH range of 5.8 to 6.0.[Conclusion]It is found that LA lily'Ben-evento'is suitable as the female parent for hybridization,while Lilium leichtlinii var.maximowiczii is suitable as the male parent for hybridization.A tissue culture system for the proliferation and expansion of hybrid seedlings is established through the setting of hormone concentrations,which provides technical support for the distant hybrid breeding of LA lily in the future.关键词
麝亚百合/亚洲百合杂种系/远缘杂交/杂交亲和性/增殖扩繁Key words
LA Lily/Lilium Asiatic hybrida/distant hybridization/hybridization compatibility/prolif-eration分类
农业科技引用本文复制引用
ZHANG Min,ZHU Zhipeng,LI Yuyao,LIAO Xiaoshan,LI Yufan..麝亚百合与6个亚洲百合原种及栽培品种杂交亲和性及杂种后代增殖技术研究[J].湖南生态科学学报,2025,12(4):29-35,7.基金项目
湖南省自然科学基金区域联合基金项目(2025JJ70064) （2025JJ70064）
