聊城大学学报（自然科学版）2026，Vol.39Issue(1)：106-117,12.DOI:10.19728/j.issn1672-6634.2025010020
辅酶Q10纳米乳的工艺优化及其抗氧化性与体内生物利用率的研究
Optimization of preparation process of coenzyme Q10 nanoemulsion and investigation of its antioxidant activity and bioavailability
摘要
Abstract
This study aims to enhance the antioxidant activity and bioavailability of Coenzyme Q10(CoQ10)by preparing CoQ10 nanoemulsion using medium chain triglycerude as the oil phase,soybean phospholipid as the surfactant,and Tween-80 as the co-surfactant.The preparation process of CoQ10 nanoemulsion was optimized using Box-Behnken response surface methodology.Also,the physicochemical properties,stability,antioxidant activity,and bioavailability of the CoQ10 nanoemulsion were investiga-ted.The results show that the optimal conditions for CoQ10 nanoemulsion were 2 g of medium chain trig-lycerude,5.353 g of soybean phospholipid,and 1 g of Tween-80.In this case,the average particle size,polydispersity index,zeta potential,and encapsulation efficiency of the CoQ10 nanoemulsion were(72.9±0.32)nm,0.25±0.034,(-34.9±1.97)mV,and(97.6±0.49)%,respectively.Transform infrared spectroscopy results show that CoQ10 was completely encapsulated in the nanoemulsion.Moreover,the result of Transmission Electron Microscopy indicates that the CoQ10 nanoemulsion were round,non-ag-gregated,and evenly dispersed in the system.The stability experiments demonstrate that CoQ10 nanoe-mulsion displayed better centrifugal stability,ionic stability,and storage stability.Additionally,com-pared to CoQ10 suspensions,the DPPH free radical,ABTS free radical,and hydroxyl free radical scaven-ging rates of CoQ10 nanoemulsion was increased by 79.1%,93.1%and 72.2%,respectively.The blood concentration of CoQ10 in rats for the optimized CoQ10 nanoemulsion was 0.767 Pg/mL,which was 1.405 times that of CoQ10 oil.This study could provide data support for the development of Coenzyme CoQ10 nanoemulsion,and lay the foundation for its application in health food and biomedicine fields.关键词
辅酶Q10/纳米乳液/响应面优化/抗氧化性/生物利用率Key words
coenzyme Q10/nanoemulsion/response surface optimization/antioxidant property activity/bioavailability分类
化学化工引用本文复制引用
YANG Yang,SHI Ruijie,LI Mingzhong,XIAO Aiai,WU Hengqian,CAI Jinyi,WANG Zhengping..辅酶Q10纳米乳的工艺优化及其抗氧化性与体内生物利用率的研究[J].聊城大学学报（自然科学版）,2026,39(1):106-117,12.基金项目
山东省外专双百计划(WSR2023087)资助 （WSR2023087）
