昆明医科大学学报2025，Vol.46Issue(12)：49-57,9.DOI:10.12259/j.issn.2095-610X.S20251206
γ-氨基丁酸A型受体的激活减轻氧糖剥夺/复氧诱导的星形胶质细胞损伤
Activation of γ-Aminobutyric Acid Type A Receptor Attenuates Oxygen-glucose Deprivation/Reoxygenation-induced Astrocyte Injury
摘要
Abstract
Objective To investigate the protective role and potential mechanisms of γ-aminobutyric acid type A receptor(GABA_AR)against oxygen-glucose deprivation/reoxygenation(OGD/R)-induced astrocyte injury.Methods C8-D1A astrocytes were used to establish an OGD/R injury model,which was randomly divided into four groups:the normal control group(NC),the OGD/R model group,the GABA_AR agonist CL218872 intervention group(OGD/R+CL218872),and the inhibitor MRK-016 intervention group(OGD/R+MRK-016).Cell proliferation was assessed by EdU staining,apoptosis was analyzed by flow cytometry,GABA_AR and glial fibrillary acidic protein(GFAP)expression were examined by immunofluorescence staining,and brain-derived n eurotrophic factor(BDNF)expression was determined by Western blotting.Results Compared with the OGD/R group,the OGD/R+CL218872 group showed significantly increased cell proliferation rate(P<0.05),markedly decreased apoptosis rate(P<0.01),upregulated expression of both GABA_AR and GFAP(P<0.05)significantly elevated BDNF protein expression level(P<0.05),and significantly reduced levels of inflammatory factors IL-6(P<0.01)and TNF-α(P<0.05).The OGD/R+MRK-016 group showed opposite changes in all parameters.Conclusion GABA_AR activation can attenuate OGD/R-induced astrocyte injury by promoting astrocyte proliferation,inhibiting apoptosis and inflammatory response,and upregulating the expression of BDNF and GFAP.关键词
γ-氨基丁酸A型受体/星形胶质细胞/缺糖缺氧/复糖复氧/突触重塑/细胞凋亡Key words
γ-aminobutyric acid type A receptor/Astrocytes/Oxygen-glucose deprivation/reoxygenation/Synaptic remodeling/Apoptosis分类
医药卫生引用本文复制引用
HE Hongxia,CHEN Tingting,HUANG Fei,LIU Junwu,ZHOU Hongli,XU Lei..γ-氨基丁酸A型受体的激活减轻氧糖剥夺/复氧诱导的星形胶质细胞损伤[J].昆明医科大学学报,2025,46(12):49-57,9.基金项目
四川省卫生健康委员会科技项目(23LCYJ007) （23LCYJ007）
