吉林农业大学学报2025，Vol.47Issue(6)：970-976,7.DOI:10.13327/j.jjlau.2021.1507
玉米自交系陈种子DNA甲基化变异的MSAP分析
MSAP Analysis of DNA Methylation Variation in Aged seeds of Maize Inbred Lines
摘要
Abstract
In order to add new content to the study of seed aging theory,DNA methylation variation of aged maize seeds was studied.In the trial,aged seeds of six maize inbred lines were selected as ex-perimental materials in comparison with new seeds of the same set of lines.DNA methylation varia-tion at the 5′-CCGG sites was analyzed by the MSAP marker.The results showed that aged maize in-bred line seeds have undergone varying degrees of changes in DNA methylation levels and patterns,and the levels of DNA methylation at 5′-CCGG sites of the aged maize inbred lines either increased or decreased,with the respective percentage ranging from 0.24%to 4.03%for the increase and from 1.05%to 1.22%for the decrease,respectively.Three types of DNA methylation variation patterns were identified in the aged seeds.The total percentage of the four patterns of single-strand CHG hemimethylation variation ranged from 5.45%to 10.42%.The percentage of the four patterns of double-strand CG full methylation variation in the DNA of the aged seeds ranged from 4.26%to 10.29%.The total percentage of simultaneous variation in CG and CHG methylation in DNA of the aged seeds ranged from 3.66%to 5.69%.According to χ2 test,there was an extremely significant cor-relation between DNA methylation variation pattern and different maize inbred lines,among which the percentage of DNA methylation variation in the aged seeds of inbred line PHJEV was the highest,reaching 77.24%.关键词
玉米/种子/DNA甲基化/MSAP/老化Key words
maize/seed/DNA methylation/MSAP/aging分类
农业科技引用本文复制引用
ZHAO Xinxin,BAI Rujin,WANG Simin,SUN Mingchun..玉米自交系陈种子DNA甲基化变异的MSAP分析[J].吉林农业大学学报,2025,47(6):970-976,7.基金项目
国家自然科学基金青年基金项目(31200231),吉林省科技发展计划项目(20200201121JC),吉林农业大学国家级大学生科技创新项目(2020) （31200231）
