昆明医科大学学报2025，Vol.46Issue(12)：75-83,9.DOI:10.12259/j.issn.2095-610X.S20251209
p16对胆囊癌细胞周期及脂肪酸代谢的影响
Effects of p16 on the Cell Cycle and Fatty Acid Metabolism of Gallbladder Cancer Cells
摘要
Abstract
Objective To verify the expression pattern of p16 at the clinical and cellular levels,and further explore the effects of p16 on the cell cycle and fatty acid metabolism of human gallbladder cancer(GBC)cells.Methods The gallbladder specimens were collected from 60 patients who underwent cholecystectomy at the Second Affiliated Hospital of Kunming Medical University from January 2021 to December 2022.Among them,30 specimens were from cases of gallstones with cholecystitis,and 30 specimens were from gallbladder cancer(GBC).Real-time fluorescent quantitative reverse transcription PCR(RT-qPCR)was used to detect p16 mRNA levels in GBC samples and cholecystitis samples.Immunohistochemical staining was performed to detect p16 protein expression levels in GBC samples and cholecystitis samples.CCK-8 assay was used to detect the proliferative capacity of stably transfected cell lines.Flow cytometry was employed to detect apoptosis and cell cycle progression in tumor cells.RT-qPCR was used to detect the expression of peroxisome-related genes,mitochondrial oxidation-related genes,and fatty acid synthesis-related genes in cancer cells with low p16 expression.Results The e xpression of p16 mRNA and p16 protein in GBC was significantly higher than that in chronic cholecystitis(P<0.05).After p16 downregulation,the apoptosis rate of tumor cells decreased,the proportion of cells in G0/G1 phase decreased,the S phase proportion increased,and cell division accelerated,with statistically significant differences(P<0.000 1).After p16 downregulation,most related genes in GBC were significantly upregulated,with statistically significant differences(P<0.05).Conclusions p16 is significantly highly expressed in GBC.p16 can inhibit the progression of GBC cell cycle.p16 may affect the occurrence and development of GBC cells through regulating the pathway of fatty acid metabolism.关键词
p16/胆囊癌/细胞周期/脂肪酸代谢/肿瘤Key words
p16/Gallbladder cancer/Cell cycle/Fatty acid metabolism/Tumor分类
医药卫生引用本文复制引用
MO Yongli,WEI Xiaoping,YU Henghai,LIU Rui..p16对胆囊癌细胞周期及脂肪酸代谢的影响[J].昆明医科大学学报,2025,46(12):75-83,9.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项(202201AY070001-116,202301AY070001-261) （202201AY070001-116,202301AY070001-261）
云南省教育厅科学研究基金(2022J0218) （2022J0218）
