吉林农业大学学报2025，Vol.47Issue(6)：1020-1028,9.DOI:10.13327/j.jjlau.2021.1624
樟子松不同病斑等级针叶内生真菌多样性及群落结构
Diversity and Community Structure of Endophytic Fungi in Needles of Pinus sylvestris var.mongolica with Different Disease Spot Grades
摘要
Abstract
Using high-throughput sequencing technology,the endophytic fungi in needles of Pinus sylvestris var.mongolica were sequenced and analyzed by bioinformatics,the Funguild database was used for fungal annotation,and the correlation between fungi was analyzed according to Spearman correlation coefficient.When the lesion length reached 1/3 of the needle length,the diversity index of endophytic fungi in needles was the lowest,and the abundance of Cyclaneusma was the highest.The dominant fungi in the six groups were Dothistroma(23.46%),Dothistroma(30.02%),Cyclaneusma(57.19%),Cyclaneusma(26.55%),Cyclaneusma(46.51%)and Cenangium(15.30%).According to PCoA results,the needles of Pinus sylvestris var.mongolica were infected by pathogens,the commu-nity structure of endophytic fungi was constantly changing,and the differences in the community structure of endophytic fungi in needles were significant.When the lesion length reached 1/3 of the needle length,the most fungi of pathotroph were found in needles(87.56%),and when the lesion length passed 1/3 of the needle length,the abundance of fungi of saprotroph increased.The diversity and community structure of endophytic fungi in the needles of Pinus sylvestris var.mongolica changed with the deepening of the disease degree.When the lesion length reached 1/3 of the needle length,the diversity of endophytic fungi was the lowest,and the diversity of endophytic fungi in-creased with the increase of lesion length.After the needles of Pinus sylvestris var.mongolica were in-fected by pathogens,the differences in the community structure of endophytic fungi in needles were significant.The abundance of fungi of pathotroph and fungi of saprotroph in needles were higher,and fungi of symbiotroph were rare,to explore and analyze the diversity and community structure of endo-phytic fungi in Pinus sylvestris var.mongolica needles.关键词
樟子松/病斑等级/针叶内生真菌/多样性/群落结构Key words
Pinus sylvestris var.mongolica/disease spot grade/coniferous endophytic fungi/diver-sity/community structure分类
农业科技引用本文复制引用
WANG Zheng,SHAO Peng,ZHONG Siwen,SONG Xiaoshuang,LIU Yalong,MA Ling,DENG Xun..樟子松不同病斑等级针叶内生真菌多样性及群落结构[J].吉林农业大学学报,2025,47(6):1020-1028,9.基金项目
国家自然科学基金项目(31670649,31700564,31200484) （31670649,31700564,31200484）
