精准医学杂志2025，Vol.40Issue(6)：471-475,5.DOI:10.13362/j.jpmed.202540106
浮针疗法对慢性压迫性坐骨神经损伤模型大鼠坐骨神经修复及炎症反应的影响
Effect of Fu's acupuncture therapy on sciatic nerve repair and inflammatory response in rats with chronic constriction injury of the sciatic nerve
摘要
Abstract
Objective To investigate the effect of Fu's acupuncture therapy on sciatic nerve repair and inflammatory re-sponse in rats with chronic constriction injury of the sciatic nerve.Methods A total of 40 adult male Sprague-Dawley rats were randomly divided into group A(control group),group B(model group),group C(traditional acupuncture therapy group),and group D(Fu's acupuncture therapy group).The rats in group A were not given any treatment,while those in groups B,C,and D were given ligation of the sciatic nerve in the right hind limb to establish a rat model of chronic constriction injury of the sciatic nerve.On days 8,10,12,and 14 of modeling,the rats in groups C were given traditional acupuncture therapy,and those in group D were given Fu's acupuncture therapy.Von Frey filaments were used to measure mechanical pain threshold on days 1,3,5,7,8,10,12,and 14 of modeling.On day 15,serum samples and sciatic nerve samples of the right hind limb were collected from each group;ELISA was used to measure the serum levels of prostaglandin E2(PGE2)and interleukin-1β(IL-1β),while HE staining was used to observe the morphology of the sciatic nerve.Results The individual effect analysis showed that there was a signifi-cant difference in mechanical pain threshold at different time points between groups(F=11.76-668.60,P＜0.05).Comparison be-tween groups showed that on days 3-14 of modeling,group A had a significantly higher mechanical pain threshold than groups B,C,and D;on days 10-14 of modeling,group B had a significantly lower mechanical pain threshold than groups C and D;on days 12 and 14 of modeling,group D had a significantly higher mechanical pain threshold than group C(F=60.89-1 050.94,P＜0.05).ELISA showed that compared with group A,group B had significant increases in the serum levels of PGE2 and IL-1β;compared with group B,groups C and D had significant reductions in these indicators;compared with group C,group D had significant reduc-tions in these indicators(F=169.39,110.05,q=3.23-20.93,P＜0.05).HE staining showed that the rats in group B had severe nerve fiber necrosis,edema,axonal shrinkage,and lymphocyte infiltration of the sciatic nerve,while those in groups C and D had a clear struc-ture of the epineurium of the sciatic nerve,with no abnormalities in the perineurial sheath or Schwann cells,hyperpla-sia,or in-flammatory cell infiltration;the rats in group C had myelin swelling and axonal shrinkage/loss of the sciatic nerve,which were oc-casionally observed in group D.Conclusion Fu's acupuncture therapy can alleviate inflammatory response and promote nerve repair,thereby showing great advantages in the treatment of sciatic neuropathic pain.关键词
浮针疗法/坐骨神经痛/痛阈/地诺前列酮/白细胞介素1β/治疗结果/疾病模型,动物Key words
Fu's acupuncture therapy/Sciatica/Pain threshold/Dinoprostone/Interleukin-1 beta/Therapeutic out-come/Disease models,animal分类
医药卫生引用本文复制引用
SUN Yarong,FANG Huiqin,ZANG Yunhua..浮针疗法对慢性压迫性坐骨神经损伤模型大鼠坐骨神经修复及炎症反应的影响[J].精准医学杂志,2025,40(6):471-475,5.基金项目
深圳市宝安区医学会医疗卫生科研项目(BAYXH2024041) （BAYXH2024041）
