精准医学杂志2025，Vol.40Issue(6)：486-491,6.DOI:10.13362/j.jpmed.202540108
抗菌肽RIKAHFKF的合成、筛选及体外抗菌活性评价
Synthesis,screening,and evaluation of the in vitro antimicrobial activity for the antimicrobial pep-tide RIKAHFKF
LI Jianren 1XU Ziyuan 1CHEN Yingxin 1KOU Zhaojun 1MA Xuezhen1
作者信息
- 1. Qingdao Medical College of Qingdao University,Qingdao 266042,China
- 折叠
摘要
Abstract
Objective To synthesize and screen for an antimicrobial peptide(AMP)with strong in vitro antibacterial ac-tivity.Methods The AMP sequence was designed,and a mixture(peptide library)consisting of four similar peptide chains was synthesized.The mixture was incubated with a bacterial cell membrane chromatographic stationary phase,and changes in peak area were detected using high-performance liquid chromatography(HPLC)to screen for the target AMP.The minimum inhibitory con-centration(MIC)and antimicrobial resistance of the target AMP were determined using the agar plate method.The cytocompati-bility of the target AMP was assessed by the tetrazolium salt colorimetric assay(MTT method).Results HPLC results showed that the peak area change rates of the solution after mixing AMP RIKAHFKF with Staphylococcus aureus and Escherichia coli cell membrane chromatographic stationary phases were significantly higher than those of the blank control(F=15 720.00,tSLD=161.40,112.70,P＜0.05).MIC testing showed that the MIC values of AMP RIKAHFKF against Staphylococcus aureus and Esc-herichia coli were 0.060 and 0.100 g/L,respectively.Resistance testing demonstrated that after10 generations of co-incubation with AMP RIKAHFKF,the growth of Staphylococcus aureus and Escherichia coli was completely inhibited.MTT assay results that compared to treatment with 0.1 g/L AMP RIKAHFKF,there was no significant change in the survival rate of L929 cells trea-ted with 0.2 or 0.4 g/L AMP RIKAHFKF(P＞0.05),whereas treatment with 0.6,0.8,or 1.0 g/L AMP RIKAHFKF significant-ly reduced cell viability(F=7.95,tLSD=3.00-18.55,P＜0.05).Conclusion AMP RIKAHFKF exhibits effective inhibitory activity against Staphylococcus aureus and Escherichia coli,including antibiotic-resistant strains,and demonstrates good cytocom-patibility.关键词
抗菌肽类/色谱法/固态合成技术/抗药性,细菌/微生物敏感性试验Key words
Antimicrobial peptides/Chromatographic method/Solid state synthesis technology/Drug resistance,bacte-ria/Microbial sensitivity test分类
医药卫生引用本文复制引用
LI Jianren,XU Ziyuan,CHEN Yingxin,KOU Zhaojun,MA Xuezhen..抗菌肽RIKAHFKF的合成、筛选及体外抗菌活性评价[J].精准医学杂志,2025,40(6):486-491,6.
