精准医学杂志2025，Vol.40Issue(6)：492-498,7.DOI:10.13362/j.jpmed.202540113
诗碧曼精华液对良性前列腺增生的影响及其机制
Effect of Shi-Bi-Man nourishing essence on benign prostatic hyperplasia and its mechanism
摘要
Abstract
Objective To investigate the effect of Shi-Bi-Man nourishing essence(SBM)on benign prostatic hyperplasia(BPH)and its mechanism.Methods A total of 21 male ICR mice aged 6-8 weeks were randomly divided into BPH group,SBM treatment group,and control group(CON group),with 7 mice in each group.The mice in the BPH group and the SBM treat-ment group were given subcutaneous injection of testosterone propionate every day to establish a model of BPH,and those in the CON group were given subcutaneous injection of an equal volume of soybean oil.Since day 2 of modeling,SBM was rubbed on the head and back of mice in the SBM group every day,and an equal volume of normal saline was rubbed on the head and back of mice in the CON group and the BPH group.After 31 consecutive days of treatment,serum and prostate tissue samples were collected,and the wet weight of prostate was measured to calculate prostate index;HE staining was used to observe histomorphological chan-ges of the prostate;ELISA was used to measure the serum levels of dihydrotestosterone(DHT),testosterone,estradiol(E2),and prostatic acid phosphatase(PAP).The human BPH epithelial cell line(BPH-1)was treated with SBM,and the BPH-1 cells treated with an equal volume of phosphate-buffered saline(PBS)were established as the control group.CCK-8 assay was used to evaluate cell proliferation rate;transmission electron microscopy and immunofluorescent staining were used to measure the number of autophagosomes in BPH-1 cells;RT-qPCR and Western blot were used to measure the expression levels of molecules associated with cell proliferation,autophagy,and glycolysis.Results Compared with the BPH group,the SBM group had a significant re-duction in prostate index,significant improvements in pathological changes such as prostatic hyperplasia and inflammatory infiltra-tion,significant reductions in the serum levels of acid phosphatase,testosterone,DHT,and PAP,and a significant increase in the serum level of E2(F=4.96-1 439.00,q=4.02-51.87,P＜0.05).Compared with the BPH-1 cells treated with PBS,the BPH-1 cells treated with SBM had a significant reduction in proliferation rate,significant reductions in the relative mRNA expression level of Ki67 and the relative mRNA and protein expression levels of PCNA,and a significant increase in the number of autophago-somes,as well as significant increases in the relative mRNA expression levels of ATG7,ATG12,and ATG16L1 and the relative mRNA and protein expression levels of ATG5,Beclin-1,HK2,PKM2,and ALDOA(F=206.00-27 234.00,q=16.99-325.50,t=8.73-82.28,P＜0.05).Conclusion SBM can alleviate BPH by activating glycolysis and enhancing autophagy in prostate epithe-lial cells.关键词
前列腺增生/诗碧曼精华液/植物药疗法/自噬/糖酵解Key words
Prostatic hyperplasia/Shi-Bi-Man nourishing essence/Phytotherapy/Autophagy/Glycolysis分类
医药卫生引用本文复制引用
PENG Chen,ZHAO Yue,WANG Tingting..诗碧曼精华液对良性前列腺增生的影响及其机制[J].精准医学杂志,2025,40(6):492-498,7.基金项目
国家自然科学基金专项项目(T2341015) （T2341015）
