精准医学杂志2025，Vol.40Issue(6)：511-515,521,6.DOI:10.13362/j.jpmed.202540059
香叶木素对大鼠肝纤维化的影响及其机制
Effect of diosmetin on rats with liver fibrosis and its mechanism
摘要
Abstract
Objective To investigate the effect of diosmetin on rats with liver fibrosis and its mechanism.Methods A total of 60 specific pathogen-free male Sprague-Dawley rats were randomly divided into groups A,B,C,D,E,and F,with 10 rats in each group.All rats except those in group A were used to establish a model of liver fibrosis induced by carbon tetrachloride(CCl4)for 10 weeks.Since week 11,the rats in groups D,E,and F were given diosmetin by gavage at a dose of 50.0,25.0,and 12.5 mg/kg,respectively,those in group C were given 25.0 mg/kg silybin by gavage,and those in groups A and B were given an equal volume of normal saline,once a day for 6 weeks.HE staining was used to observe liver histomorphology,and Masson stai-ning was used to observe the degree of liver fibrosis in each group;the fluorescent probe method was used to measure the level of reactive oxygen species(ROS)in rat liver tissue;RT-qPCR was used to measure the relative mRNA expression levels of nicoti-namide adenine dinucleotide phosphate oxidase type 2(NOX2),nucleotide-binding oligomerization domain-like receptor 3(NLRP3),and interleukin-1β(IL-1β)in rat liver tissue,and Western blot(WB)was used to measure the relative protein expres-sion levels of NOX2,NLRP3,and IL-1β in rat liver tissue.Results Compared with group A,group B showed structural da-mage of hepatic lobules,hepatocyte degeneration and necrosis,and pseudolobule formation,with inflammatory infiltration and ex-tensive fibrous tissue proliferation,and compared with group B,groups C,D,E,and F had significant alleviation of the above pathological changes,with the highest degree of alleviation in group D.Compared with group B,groups C,D,E,and F had signi-ficantly lower ROS fluorescence intensity and relative mRNA and protein expression levels NOX2,NLRP3,and IL-1β(F=93.516-142.879,t=5.270-31.333,P＜0.05),and compared with group C,group D had significantly lower indicators(t=2.572-8.825,P＜0.05)and group F had significantly higher indicators(t=2.774-16.488,P＜0.05).Conclusion Diosmetin can alleviate liver pathological changes and fibrosis in rats induced by CCl4,possibly by inhibiting the NOX2 signaling pathway,re-ducing the level of ROS,and downregulating the expression of NLRP3 and IL-1β in liver tissue.关键词
香叶木素/肝纤维化/NADPH氧化酶类/NLR家族,热蛋白结构域包含蛋白3/四氯化碳Key words
Diosmetin/Liver fibrosis/NADPH oxidases/NLR family,pyrin domain-containing 3 protein/Carbon tet-rachloride分类
医药卫生引用本文复制引用
TAN Lixia,HUA Jing,YAN Chunxiao,WANG Hangyu,DONG Yong,WEI Shutang..香叶木素对大鼠肝纤维化的影响及其机制[J].精准医学杂志,2025,40(6):511-515,521,6.基金项目
开封市科技攻关计划(农业社发领域)项目(190-3099) （农业社发领域）
