精准医学杂志2025，Vol.40Issue(6)：516-521,6.DOI:10.13362/j.jpmed.202540111
滋肾育胎丸对人卵巢颗粒细胞焦亡的调控作用及机制
The regulatory role and mechanism of action of Zishen Yutai pills on pyroptosis of human ovarian granulosa cells
摘要
Abstract
Objective To investigate the regulatory role and mechanism of action of Zishen Yutai(ZSYT)pills on the pyroptosis of human ovarian granulosa cells.Methods A total of 30 female Sprague-Dawley rats,aged 8 weeks,were randomly divided into control group and treatment group,with 15 rats in each group.The rats in the treatment group were given ZSYT sus-pension by gavage,while those in the control group were given an equal volume of distilled water by gavage.After treatment for 7 d,blood samples were collected from the abdominal aorta to prepare control serum and serum containing ZSYT.Human ovarian granulosa cells(KGN cells)were divided into group A(control),group B(inducing pyroptosis),group C(inducing pyroptosis+5％drug-containing serum),group D(inducing pyroptosis+10％drug-containing serum),group E(inducing pyroptosis+15％drug-containing serum),group F(inducing pyroptosis+20％drug-containing serum),group G(inducing pyroptosis+25％drug-containing serum).The cells in groups B-G were treated with LPS combined with nigericin in the culture wells,while those in group A were cultured in an equal volume of drug-free medium;subsequently,the cells in groups C-G were added with serum containing ZSYT at the corresponding concentrations,and those in groups A and B were added with 20％control serum;all groups were cultured in an incubator.CCK-8 assay was used to measure cell viability in groups A-G;groups A,B,and F were measured in terms of the level of lactate dehydrogenase(LDH)in supernatant and estradiol(E2)synthesis capacity;RT-qPCR and Western blotting were used to measure the mRNA and protein expression levels of pyroptosis-related molecules and the key estrogen synthe-sis enzyme CYP19a1 in groups A,B,and F.Results CCK-8 assay showed that compared with group B,groups E-G had a significant increase in cell viability(F=80.08,tLSD=7.35-12.57,P＜0.05).Compared with group B,group F had significant re-ductions in the level of LDH in supernatant,the relative mRNA expression levels of NLRP3,GSDMD,IL-1β,IL-18,and caspase-1 in cells,and the relative protein expression levels of NLRP3,GSDMD,GSDMD-N,and caspase-1(F=21.66-319.40,tLSD=3.88-13.65,P＜0.05),as well as significant increases in E2 synthesis capacity and the relative mRNA and protein expres-sion levels of CYP19a1 in cells(F=72.26-151.01,tLSD=2.60-8.38,P＜0.05).Conclusion ZSYT pills reduce the level of py-roptosis in ovarian granulosa cells and restores their E2 synthesis capacity by inhibiting the NLRP3/GSDMD pathway.关键词
滋肾育胎丸/中草药/早发性卵巢功能不全/细胞焦亡/雌激素类Key words
Zishen Yutai Pills/Drugs/Premature ovarian insufficiency/Pyroptosis/Estrogens分类
医药卫生引用本文复制引用
LI Juan'e,ZHAO Jun,DU Xin..滋肾育胎丸对人卵巢颗粒细胞焦亡的调控作用及机制[J].精准医学杂志,2025,40(6):516-521,6.基金项目
国家自然科学基金青年项目(81904244) （81904244）
山东省"十二五"中医药科技发展计划项目(2011-218) （2011-218）
北京大学人民医院学术新星项目(RS2021-15) （RS2021-15）
