精准医学杂志2025，Vol.40Issue(6)：522-526,531,6.DOI:10.13362/j.jpmed.202540116
耐碳青霉烯类霍氏肠杆菌分离株的基因型及其碳青霉烯酶基因水平转移情况的单中心研究
A single-center study on the Genotype and horizontal carbapenemase gene transfer situation of car-bapenem-resistant Enterobacter hormaechei strains
摘要
Abstract
Objective To investigate the genotype,homology,and horizontal carbapenemase gene transfer situation of carbapenem-resistant Enterobacter hormaechei isolates from a hospital,and to provide a basis for formulating prevention and con-trol measures for this bacterium.Methods Eleven non-repeating Enterobacter cloacae strains were collected from the children who were hospitalized in a hospital from September 2016 to May 2017 and were numbered as Q1-Q11,and Illumina and Nanopore platforms were used for whole-genome sequencing.Pulsed-field gel electrophoresis(PFGE)was used to analyze the homology be-tween strains,and liquid conjugation assay and S1 nuclease digestion followed by PFGE were used to analyze the carbapenemase gene transfer ability of the strains.Results The average nucleotide identity analysis showed that among the eleven strains,strain Q10 was identified as Enterobacter bugandensis,and the remaining ten strains were identified as Enterobacter hormaechei.All eleven strains carried the blaNDM-1 gene and had five multi-locus sequence genotypes,among which ST177 was the main geno-type and accounted for 63.6％(7/11).The results of PFGE showed that the Q4-Q8 strains of Enterobacter hormaechei had a Dice similarity coefficient of 100.0％,all of which had the ST177 type and carried the same drug-resistance gene.The bioinformatics analysis showed that the blaNDM-1 gene carried by strain Q4 was located on the plasmid.Liquid conjugation assay showed that the plasmid carrying blaNDM-1 can be delivered to E.coliJ53AziR.Conclusion Enterobacter hormaechei ST177 harboring the bla NDM-1 gene is the pathogen causing the outbreak of nosocomial infection in neonates,and the blaNDM-1 gene can be transmitted be-tween different species through mediation by plasmids.关键词
耐碳青霉烯类肠杆菌科/阴沟肠杆菌/交叉感染/blaNDM-1/质粒/疾病暴发流行/婴儿,新生Key words
Carbapenem-resistant enterobacteriaceae/Enterobacter cloacae/Cross infection/blaNDM-1/Plasmids/Di-sease outbreaks/Infant,newborn分类
医药卫生引用本文复制引用
TIAN Zhe,JIANG Meiyan,LIU Zhenzhen,DONG Dan-dan,WANG Bin,GAO Zhenliang..耐碳青霉烯类霍氏肠杆菌分离株的基因型及其碳青霉烯酶基因水平转移情况的单中心研究[J].精准医学杂志,2025,40(6):522-526,531,6.基金项目
青岛大学附属医院"临床医学+X"科研项目(2019-3399) （2019-3399）
