现代畜牧兽医Issue(12)：7-11,5.DOI:10.20154/j.cnki.issn1672-9692.2025.12.002
鸡传染性法氏囊病病毒VP2蛋白在重组杆状病毒系统中的表达及免疫原性研究
Expression and immunogenicity of the VP2 protein of infectious bursal disease virus in a recombinant baculovirus system
Shen Haoyue 1Xia Wei 1Wan Rongjie 1Wang Feng 1Zuo Ronglin 1Lin Yan 1Li Sanpeng1
作者信息
- 1. Chengdu Shiji Biological Pharmaceutical Co.,Ltd.,Sichuan Chengdu 610100
- 折叠
摘要
Abstract
This study aims to provide a reference for the subsequent development of subunit vaccines against infectious bursal disease virus(IBDV)and avian multivalent combination vaccines.The VP2 protein sequence of the IBDV-TB strain was codon-optimized,and a recombinant baculovirus stably expressing the VP2 protein was constructed.The expressed VP2 protein was formulated into an oil-emulsion vaccine and administered to specific pathogen-free(SPF)chickens to evaluate its immunogenicity.The results showed that the VP2 protein expressed by the recombinant baculovirus had a molecular weight of approximately 42 kDa and exhibited strong reactivity with IBDV-positive serum.The expressed protein maintained a stable spatial conformation and could self-assemble into virus-like particles(VLP).On day 28 post-immunization with the oil-emulsion vaccine prepared from VP2 protein,all SPF chickens showed agar gel precipitation titers of≥1∶32 in serum,and effective immune protection was provided against classical,variant,and very virulent strains.The study demonstrates that the codon-optimized VP2 protein sequence of the IBDV-TB strain can be successfully expressed via a baculovirus expression system and self-assemble into VLP.The oil-emulsion vaccine prepared from these VLP provides effective protection against multiple IBDV strains and holds potential for further development as an IBDV subunit vaccine or a component of avian multivalent vaccines.关键词
鸡传染性法氏囊病病毒/重组杆状病毒/VP2蛋白/攻毒保护Key words
Infectious bursal disease virus(IBDV)/Recombinant baculovirus/VP2 protein/Challenge protection分类
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Shen Haoyue,Xia Wei,Wan Rongjie,Wang Feng,Zuo Ronglin,Lin Yan,Li Sanpeng..鸡传染性法氏囊病病毒VP2蛋白在重组杆状病毒系统中的表达及免疫原性研究[J].现代畜牧兽医,2025,(12):7-11,5.
