现代畜牧兽医Issue(12)：27-31,5.DOI:10.20154/j.cnki.issn1672-9692.2025.12.006
红景天苷发酵液的大孔树脂分离与回收技术研究
Study on the separation and recovery technology of macroporous resin in salidroside fermentation solution
Yuan Yong 1Chen Hongfei 1Li Runquan 1Shen Ruoyao 1Liu Hong 1Fang Wei1
作者信息
- 1. Jihua Laboratory,Guangdong Foshan 528200
- 折叠
摘要
Abstract
The study aims to optimize the adsorption and desorption process of salidroside using macroporous resin to achieve efficient separation and purification of salidroside.The experiment first screened suitable macroporous resins through static adsorption,followed by dynamic adsorption to determine the optimal pH,concentration,and flow rate of the sample solution.Stepwise desorption was employed to identify the appropriate eluent concentration.The samples obtained from the previous steps were used to establish a standard curve,measure recovery rate and purity,and the structure of salidroside was verified using high-performance liquid chromatography-mass spectrometry(HPLC-MS),and nuclear magnetic resonance(NMR).The results showed that using macroporous resin LX-32 under optimal conditions(sample solution pH 6.0,salidroside content 0.4 g/L,flow rate 1.0 BV/h,eluted with 20%ethanol at a flow rate of 4.0 BV/h),the recovery rate of salidroside reached 92.5%with a purity of 99.8%.The study demonstrates that the established macroporous resin separation and recovery process for salidroside extraction and purification offers significant advantages.It not only improves the efficiency of separation and purification but also reduces production costs.This provides reliable technical support for the industrial production of salidroside and promotes its application and development in the pharmaceutical field.关键词
红景天苷/大孔树脂/吸附分离/解吸洗脱/纯化回收Key words
Salidroside/Macroporous resin/Adsorption separation/Desorption and elution/Purification and recovery分类
农业科技引用本文复制引用
Yuan Yong,Chen Hongfei,Li Runquan,Shen Ruoyao,Liu Hong,Fang Wei..红景天苷发酵液的大孔树脂分离与回收技术研究[J].现代畜牧兽医,2025,(12):27-31,5.
