林产化学与工业Issue(6)：53-62,10.DOI:10.20195/j.issn.0253-2417.2024199
枸杞果渣总黄酮低共熔溶剂提取工艺优化及其活性成分分析
Deep Eutectic Solvent Extraction of Total Flavonoids From Lycium barbarum Pomace:Optimization and Active Composition Analysis
摘要
Abstract
Using choline chloride(ChCl)as a hydrogen bond acceptor,thirty-six deep eutectic solvent(DES)were synthesized and compared with the conventional solvent ethanol(EtOH)for the extraction of total flavonoids(TF)from Lycium barbarum pomace.The optimal DES system was determined based on extraction yield,and the ultrasound-assisted extraction conditions for TF were optimized using single-factor experiments and response surface methodology.The flavonoid compounds in L.barbarum pomace were quantified using liquid chromatography-tandem mass spectrometry(LC-MS/MS),and the antioxidant activity of the extracts was evaluated.The results indicate that using a DES composed of choline chloride and 1,2-butanediol/triethylene glycol at a molar ratio of 1∶1.35 as the extraction solvent,with14%water content of DES,a liquid-to-material ratio of 24∶1(mL∶g),an ultrasonic extraction temperature of 45℃,and an ultrasonic extraction time of 49 min,achieved a TF yield of 13.104 mg/g.The yield was significantly higher than the3.272 mg/g,which was obtained through traditional ethanol extraction.Five flavonoids of kaempferol,chrysin,spiraeoside,tiliroside,and phloretin were identified in the crude extract of DES.None of them was detected in the crude extract of 60%ethanol.Notably,the contents of isorhamnetin and apigenin were found to be 4.21 and 3.88 times higher,respectively,compared to those in the crude extract of 60%ethanol.Furthermore,within a certain mass concentration range,the scavenging rate of superoxide anions and hydroxyl radicals as well as the total antioxidant capacity of the DES extract generally surpasses that of the 60%ethanol extract.关键词
枸杞果渣/低共熔溶剂/总黄酮/LC-MS/MS/抗氧化活性Key words
Lycium barbarum pomace/deep eutectic solvent/total flavonoids/LC-MS/MS/antioxidant activity分类
化学化工引用本文复制引用
CHEN Li,LIANG Qinlan,WANG Hui,ZHENG Weihua,YANG Xiaojun,MA Lei..枸杞果渣总黄酮低共熔溶剂提取工艺优化及其活性成分分析[J].林产化学与工业,2025,(6):53-62,10.基金项目
新疆维吾尔自治区公益性基本科研业务费项目(KY2023033) （KY2023033）
