林产化学与工业Issue(6)：79-88,10.DOI:10.20195/j.issn.0253-2417.2024204
慈竹秆多糖加压热水提取工艺优化及其抗氧化活性评价
Optimization of Pressurized Hot Water Extraction of Bambusa emeiensis Culm Polysaccharides and Evaluation of Their Antioxidant Activity
摘要
Abstract
Polysaccharides were extracted from the culms of Bambusa emeiensis L.C.Chia&H.L.Fung using a pressurized hot water extraction(PHWE)method.The extraction conditions were optimized by response surface methodology,the antioxidant activity of the polysaccharides was evaluated by free radical scavenging test,and the structure of the purified polysaccharide was analyzed by gel permeation chromatography(GPC)and infrared spectroscopy.The results indicated that extraction temperature significantly affected the total sugar yield,while extraction time significantly influenced the degree of polysaccharide degradation(polysaccharide/total sugar ratio).The optimal extraction conditions were determined as follows:extraction temperature of 139.5℃,extraction time of 32 min,and a liquid-to-solid ratio of 15.5∶1(mL∶g).Under these conditions,the yield of total sugars from B.emeiensis culms was 1.39%,the polysaccharide yield was 0.81%,and the polysaccharide/total sugar ratio was 0.57.The polysaccharides exhibited considerable antioxidant activity,with half-maximal inhibitory mass concentration(IC50)values for scavenging DPPH·,·ABTS+,and·OH being 1.89,0.86,and 0.22 g/L,respectively.The polysaccharides were separated and purified using a DEAE-52 of cellulose column,yielding one purified polysaccharide fraction,designated PWDP-N.PWDP-N had a weight-average molecular mass of 14 484 u and a dispersity index of 1.00.It exhibited characteristic infrared absorption peaks of a neutral polysaccharide.Monosaccharide composition analysis revealed that PWDP-N was composed of eight monosaccharides,including mannose,galactose,and glucose.关键词
慈竹/竹秆多糖/加压热水提取/抗氧化活性/结构表征Key words
Bambusa emeiensis/bamboo culm polysaccharides/pressurized hot water extraction/antioxidant activity/structural characterization分类
化学化工引用本文复制引用
JIANG Wanying,YANG Yuan,LI Yunchuan,XUN Hang,XU Yuan,TANG Feng..慈竹秆多糖加压热水提取工艺优化及其抗氧化活性评价[J].林产化学与工业,2025,(6):79-88,10.基金项目
国家重点研发计划资助项目(2022YFD2200905) （2022YFD2200905）
国际竹藤中心与青神县共建竹产业博士工作站项目(206030202076703) （206030202076703）
