南阳师范学院学报2026，Vol.25Issue(1)：53-59,7.
猪圆环病毒4型Cap蛋白的原核表达及其多克隆抗体的制备
Prokaryotic Expression of the Cap Protein of Porcine Circovirus Type 4 and Preparation of Its Polyclonal Antibody
摘要
Abstract
In order to prepare polyclonal antibody against Cap protein of porcine circovirus type 4(PCV4),the Cap deleted nuclear location signal(NLS)gene of PCV4 HNU-AHG1-2019 was synthesized based on the codon bias of E.coli and cloned into prokaryotic expression plasmid pET-28a.After verification by enzyme digesting and sequencing,the recombinant positive plasmid pET-28a-Cap was transformed into E.coli BL21(DE3)com-petent cells for induced expression.Through condition optimization,it was found that under the induction of 30℃and 0.6 mmol/L IPTG,approximately 25 kDa of Cap protein was efficiently expressed in soluble form.The purified Cap protein was immunized in BALB/c mice,and the obtained polyclonal antibody had an antibody titer of up to 1∶512 000.The results of Western blot and indirect immunofluorescence(IFA)further confirmed that this antibody could specifically recognize the PCV4 Cap protein.关键词
猪圆环病毒4型/Cap蛋白/原核表达/多克隆抗体Key words
porcine circovirus type 4/Cap protein/prokaryotic expression/polyclonal antibody分类
生物科学引用本文复制引用
CAI Yingying,HAN Xueying,DENG Jiaxin,BI Zhen,YAO Lunguang,LIU Yangkun..猪圆环病毒4型Cap蛋白的原核表达及其多克隆抗体的制备[J].南阳师范学院学报,2026,25(1):53-59,7.基金项目
河南省中原科技创新领军人才项目"治疗仔猪病毒性腹泻基因工程卵黄抗体的制备与应用"(244200510040) （244200510040）
河南省科技攻关项目"猪流行性腹泻Mosaic纳米颗粒疫苗的研制与应用"(252102111026). （252102111026）
