南阳师范学院学报2026，Vol.25Issue(1)：60-69,10.
基于孟德尔随机化探索肠道菌群、血浆代谢物和弥漫性大B细胞淋巴瘤的因果关系
Exploration of Causal Relationships Between Gut Microbiota,Plasma Metabolites,and Diffuse Large B-cell Lymphoma Based on Mendelian Randomization
摘要
Abstract
The paper aims to investigate the causal relationships between gut microbiota,plasma metabolites,and diffuse large B-cell lymphoma(DLBCL)using Mendelian randomization(MR)analysis,and to explore the me-diating role of metabolites.In the study,A two-sample Mendelian randomization approach was employed,in-tegrating genome-wide association study(GWAS)data on DLBCL,473 gut microbiota taxa,and 1 400 plasma metabolites.The inverse-variance weighted(IVW)method served as the primary analysis,supplemented by MR-Egger,weighted median(WM),and other methods for causal inference.Mediation MR analysis was con-ducted to assess metabolite-mediated effects.Horizontal pleiotropy and heterogeneity tests were performed to en-sure the robustness of the findings.The results as follows:twenty-one gut microbiota taxa had significant causal associations with DLBCL,with the order Lachnospirales exhibiting the strongest pathogenic effect(OR=2.907 6,P=0.034 2),while species CAG-177 demonstrated the most notable protective effect(OR=0.596 8,P=0.000 5).Besides,forty-five metabolites and their ratios were associated with DLBCL,with the 3′,5′-cyclic adenosine monophosphate/taurocholic acid ratio(OR=1.718 9,P=0.005 1)and cortisone(OR=1.640 7,P=0.016 7)identified as major risk factors.Mediation analysis revealed that the 3′,5′-cyclic adenosine mono-phosphate/taurocholic acid ratio mediated 23.25%of the effect of Lachnospirales on DLBCL,while phenylace-tylcarnitine mediated 10.81%of the effect of phylum SAR324 on DLBCL.In conclusion,the study reveals the causal participation of gut microbiota and plasma metabolites in the pathogenesis of DLBCL,and provides poten-tial biomarkers and therapeutic targets for its prevention and treatment.关键词
弥漫性大B细胞淋巴瘤/肠道菌群/血浆代谢物/孟德尔随机化/中介分析Key words
diffuse large B-cell lymphoma/gut microbiota/plasma metabolites/Mendelian randomization/medi-ation analysis分类
医药卫生引用本文复制引用
CHANG Jiahao,WANG Zongqing,SUN Zhengqiang,ZHANG Xinying,XU Qian..基于孟德尔随机化探索肠道菌群、血浆代谢物和弥漫性大B细胞淋巴瘤的因果关系[J].南阳师范学院学报,2026,25(1):60-69,10.基金项目
河南省科技厅重点研发项目"基于气血理论的益气活血法促缺血型烟雾病术后血管新生作用"(231111310900). （231111310900）
