世界科学技术-中医药现代化2025，Vol.27Issue(12)：3631-3642,12.DOI:10.11842/wst.20250804002
GEO联合孟德尔随机化探索骨关节炎发病机制
Exploring the Pathogenesis of Osteoarthritis by Combining GEO with Mendelian Randomization
摘要
Abstract
Objective To explore the pathogenesis of osteoarthritis(OA)by integrating Gene Expression Omnibus(GEO)-database analysis with Mendelian randomization.Methods The chip containing OA and synovial membrane gene data of healthy people was downloaded from GEO.After classification into control and test groups,differential genes were identified with limma and weighted gene co-expression network analysis(WGCNA).Core genes were then selected via the STRING platform and Cytoscape software.Among them,MYC proto-oncogene proteins related to core genes and with research value were screened from the whole genome sequencing data(GWAS)database as exposure data.SNPs strongly associated with MYC were used as instrumental variables,and Mendelian randomization analysis was conducted using knee OA(KOA),OA,and coxitis(COX)as outcomes data respectively.Results After screening,it was finally determined that IL1B,JUN,MYC,CD4 and PTPRC were the top five genes in terms of the number of gene interaction nodes.The MYC proto-oncogene protein and KOA were analyzed by Mendelian randomization.The OR value detected by the inverse variance weighted method(IVW method)was 1.103(95%CI:1.002-1.215),indicating a positive correlation between the two(P value=0.046),indicating that there might be a causal relationship between them.Conclusion The MYC proto-oncogene protein may be a potential risk factor for the onset of KOA.关键词
MYC原癌基因蛋白/骨关节炎/孟德尔随机化/差异基因/GEO数据库Key words
MYC proto-oncogene protein/Osteoarthritis/Mendelian randomization/Differential gene/GEO database分类
医药卫生引用本文复制引用
XU Bo,WANG Jiqing,WANG Yuqing,XU Shifen,LI Shanshan,MI Yiqun..GEO联合孟德尔随机化探索骨关节炎发病机制[J].世界科学技术-中医药现代化,2025,27(12):3631-3642,12.基金项目
国家自然科学基金委员会面上项目(82474619):基于SIRT3介导的去乙酰化调控线粒体稳态探究温针灸治疗骨关节炎的作用机制,负责人:宓轶群 （82474619）
上海市中医药(临床类)重点学科建设项目(shzyyzdxk-2024108):数智针灸学,负责人:徐世芬 （临床类）
上海市中医药传承创新发展三年行动计划(ZY(2025-2027)-3-2-1):海派中医非药物疗法推广体系建设项目,负责人:宓轶群、张洁. （ZY(2025-2027）
