上海中医药杂志2026，Vol.60Issue(1)：12-20,9.DOI:10.16305/j.1007-1334.2026.z20250323001
基于串联质谱标签的电针改善阿尔茨海默病大鼠学习记忆损伤的海马组织蛋白质组学研究
Proteomic study of hippocampal tissue on electroacupuncture‑improved learning and memory impairment in Alzheimer's disease rats based on tandem mass tag labeling
摘要
Abstract
Objective To investigate the effects of electroacupuncture(EA)on learning and memory abilities and hippocampal tissue proteome in Alzheimer's disease(AD)rats,and to explore differential proteins and regulatory pathways involved in the prevention and treatment of AD by EA.Methods Twenty-four SD rats were randomly divided into sham operation(Sham)group,Model group,and EA group,with 8 rats in each group.The AD model was established through injection of amyloid β protein 1-42 into bilateral hippocampal CA1 region.Following 7-week EA intervention,the learning and memory abilities of rats were evaluated using the Morris water maze experiment,and the recognition memory and novelty preference abilities of rats were assessed through the novel object recognition experiment.Hippocampal proteomic profiling was performed through tandem mass tag-labeling quantitative proteomics.Differential proteins underwent Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses.The key differential proteins were verified using the parallel reaction monitoring(PRM)method.Results Compared with the Sham group,the rats in the Model group exhibited significantly prolonged escape latency(P＜0.01),reduced platform crossings numbers(P＜0.01),and decreased target quadrant dwell time(P＜0.01).Compared with the Model group,the escape latency of rats in the EA group was significantly shorter than that of the Model group(P＜0.01),the number of platform crossings was significantly increased(P＜0.01),and the target quadrant dwell time was significantly prolonged(P＜0.01).There was no significant difference in swimming speed among the different groups of rats(P＞0.05).During the exploration of the same objects,there was no significant difference in the recognition index of the same objects among all groups of rats(P＞0.05);during the exploration of new objects,compared with the Sham group,the recognition index of new objects in the Model group was significantly decreased(P＜0.01),and compared with the Model group,the recognition index of novel objects in the EA group was significantly increased(P＜0.01).The proteomic analysis results showed that a total of 211 differentially expressed proteins were identified.Based on these differences,11 significant reversal target proteins were selected.KEGG enrichment results highlighted synaptic vesicle cycle and glutamatergic synapse pathways as primary regulatory mechanisms.Two key proteins were finally identified,namely protein tyrosine phosphatase non-receptor type 5(PTPN5)as well as sodium and chloride-dependent γ-aminobutyric acid transporter 3(GAT3),which were associated with the glutamatergic synaptic pathway and the synaptic vesicle pathway respectively.The PRM results indicated that compared with the Sham group,the expressions of PTPN5 and GAT3 proteins in the hippocampal tissue of the Model group were increased(P＜0.01);compared with the Model group,the expressions of PTPN5 and GAT3 proteins in the hippocampal tissue of the EA group were decreased(P＜0.01),which was consistent with the proteinomics trends.Conclusions EA may improve the learning and memory abilities of AD rats by regulating the expressions of PTPN5 and GAT3 in the hippocampal tissue.关键词
阿尔茨海默病/电针/串联质谱标签/平行反应监测/蛋白质组学/作用机制Key words
Alzheimer's disease/electroacupuncture/tandem mass tag labeling/parallel reaction monitoring/proteomics/mechanism of action引用本文复制引用
KE Chao,SHAN Shengtao,CAO Yang,TAN Yan,XIE Zhengrong,ZHOU Zhuo,YUAN Liumei,PAN Jiang,ZHANG Wei..基于串联质谱标签的电针改善阿尔茨海默病大鼠学习记忆损伤的海马组织蛋白质组学研究[J].上海中医药杂志,2026,60(1):12-20,9.基金项目
国家自然科学基金项目(81973956,82405580) （81973956,82405580）
湖南省自然科学基金项目(2025JJ60638) （2025JJ60638）
长沙市自然科学基金项目(kq2403097) （kq2403097）
