山西医科大学学报2025，Vol.56Issue(12)：1407-1415,9.DOI:10.13753/j.issn.1007-6611.2025.12.012
非法染色延胡索指标成分快速检测模型的构建及其对镇痛疗效的影响
Construction of a rapid detection model for components in illegally dyed Rhizoma Corydalis and its effect on analgesic efficacy
摘要
Abstract
Objective To establish qualitative identification models for illegally dyed Rhizoma Corydalis and rapid quantitative detection models for multiple components by means of near-infrared spectroscopy,and analyze the changes in analgesic activity of Rhizoma Corydalis caused by altered index components under different dyeing degrees.Methods Samples of Rhizoma Corydalis stained with different degrees of Auramine O were prepared.HPLC was used to simultaneously determine the contents of berberine hydrochloride,palmatine hydrochloride,and tetrahydropalmatine in Rhizoma Corydalis,and their measured values were used as modeling reference.The near-infrared spectra of all samples were collected using an integrating sphere diffuse reflectance mode.Similarity discriminant analysis was used to establish a qualitative model,and partial least squares(PLS)was used to establish a quantitative model for the components.The analgesic activity of Rhizoma Corydalis with different degrees of immersion was investigated from three aspects:acetic acid writhing reaction in mice,hot plate analgesia in mice,and oxytocin-induced dysmenorrhea rat model.Results After MSC stan-dardization,stained and unstained Rhizoma Corydalis were well distinguished in the range of 4 500-8 500 cm-1,with an internal vali-dation accuracy of 100%and an external validation accuracy of 100%.The quantitative model of tetrahydropalmatine was selected in the spectral bands of 4 000-6 000 cm-1 and 7 000-8 000 cm-1;after MSC processing,the R2,RMSECV,and RMSEP of the models were 0.984 4,0.341 2,and 0.355 6,respectively.The quantitative model of berberine hydrochloride was selected in the bands of 4 000-5 500 cm-1 and 7 000-8 000 cm-1;after SNV treatment,the R2,RMSECV,and RMSEP of the models were 0.973 5,0.513 7,and 0.548 5,respectively.The quantitative model of palmatine hydrochloride was selected with spectral bands of 5 000-6 000 cm-1 and 7 000-8 000 cm-1;after MSC treatment,the R2,RMSECV,and RMSEP of the models were 0.961 1,0.321 1,and 0.336 5,respectively.When the soaking time of Rhizoma Corydalis with Auramine O ethanol solution reached 5 min or the concentration of Aura-mine O exceeded 2%,the analgesic effect of Rhizoma Corydalis on mice was significantly reduced.When the soaking time of Rhizoma Corydalis with auramine O ethanol solution reached 10 min or the concentration of auramine O exceeded 2%,the analgesic effect of Rhizoma Corydalis on rats was significantly reduced.Conclusion Near-infrared spectroscopy achieves complete discrimination of illegally dyed Rhizoma Corydalis,and rapid detection of berberine hydrochloride,palmatine hydrochloride,and tetrahydropalmatine.When Rhizoma Corydalis is dyed with Auramine O for 5 min or longer,or at a concentration of 2%or higher,its analgesic activity is significantly reduced,thus,the dye for Rhizoma Corydalis should be strictly prohibited.关键词
延胡索/近红外光谱技术/延胡索乙素/定性分析/定量分析/镇痛活性Key words
Rhizoma Corydalis/near-infrared spectroscopy/tetrahydropalmatine/qualitative analysis/quantitative analysis/analgesic activity分类
医药卫生引用本文复制引用
WANG Lixia,XI Xiaohu,ZHAO Zhijun..非法染色延胡索指标成分快速检测模型的构建及其对镇痛疗效的影响[J].山西医科大学学报,2025,56(12):1407-1415,9.基金项目
山西省中医药管理局科研项目(2024ZYYZ007) （2024ZYYZ007）
山西省大健康产业高质量发展科研专项课题(DJKZXKT2023060) （DJKZXKT2023060）
