生物技术通报2025，Vol.41Issue(12)：106-113,8.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0654
野生稻内生菌Enterobacter hormaechei QT2在盐碱地水稻增产中的应用
Boosting Rice Yield in Saline-alkali Lands Using Enterobacter hormaechei QT2:A Wild Rice Endophytic Bacterium
摘要
Abstract
[Objective]Screening wild rice-derived endophytic bacteria with high potential for mitigating salinity-alkalinity stress,and evaluating the effects of Enterobacter hormaechei QT2 on the growth,physiology,and yield of cultivated rice under such stress conditions.This study aims to provide a theoretical basis for stabilizing rice yields and enhancing efficiency in saline-alkali lands,as well as for developing stress-resistant microbial agents.[Method]The endophytic bacterium E.hormaechei QT2 was isolated from the leaves of wild rice,and its growth-promoting characteristics and yield-enhancing effects were tested in a pot experiment(middle-heavy saline-alkaline soil)and a field validation(pH 9.3,conductivity 1 262 μS/cm).[Result]Under saline-alkali stress,inoculation of QT2 significantly improved the growth phenotype of cultivated rice(P<0.05),demonstrated the increase of plant height,root length,fresh weight and dry weight increased by 101.0%,46.1%,137.4%and 447.0%,respectively.Meanwhile,the osmotically regulated substances were significantly accumulated in the leaves of cultivated rice,and the contents of soluble sugars,soluble proteins and proline increased by 135.7%,94.0%and 205.2%,respectively.The content of malondialdehyde(MDA)reduced by 59.6%,and the membrane stability index(MSI)increased by 42.3%.The activity of antioxidant system in the leaves of the cultivated rice was significantly enhanced,and the activities of superoxide dismutase(SOD),peroxidase(POD)and the content of glutathione(GSH)increased by 57.5%,73.3%and 39.4%respectively.The photosynthesis of cultivated rice leaves was enhanced,and the contents of chlorophyll a,chlorophyll b and carotenoids increased by 30.69%,78.57%and 75.62%,respectively.In addition,strain QT2 also showed a variety of plant growth-promoting properties(nitrogen fixation,phosphorus solubilization,potassium solubilization,IAA production,ACC deaminase production and ammonia production).Field experiments further confirmed that inoculation with QT2 increased the yield of saline cultivated rice by 29.5%,and the input-output ratio of strain QT2 reached 1:46.[Conclusion]The endophytic bacterium E.hormaechei QT2 effectively alleviates the inhibitory effects of saline-alkali stress on cultivated rice by synergistically regulating osmotic balance,enhancing antioxidant defense,and improving photosynthetic efficiency,thereby significantly promoting growth and increasing yield.关键词
野生稻/内生细菌/贺氏肠杆菌/栽培稻/盐碱胁迫Key words
wild rice/endophytic bacteria/Enterobacter hormaechei/cultivated rice/saline-alkali stress引用本文复制引用
LI Jun-chen,ZHAO Xiang-yuan,WANG Fu-dong,WANG Yi-fan,ZHANG Jian-feng,WANG Shu-sheng..野生稻内生菌Enterobacter hormaechei QT2在盐碱地水稻增产中的应用[J].生物技术通报,2025,41(12):106-113,8.基金项目
吉林省科技发展项目(20220202022NC) （20220202022NC）
