山西中医药大学学报2025，Vol.26Issue(11)：1209-1215,7.DOI:10.19763/j.cnki.2096-7403.2025.11.07
不育1号方改善弱精子症小鼠生殖功能的代谢组学机制
Metabolomic mechanisms of the Buyu No.1 Formula in improving reproductive function in mice with asthenozoospermia
摘要
Abstract
Objective:To investigate the therapeutic effects of Buyu No.1 Formula(BY1F)on semen quality in glyco-side tripterygium wilfordii(GTW)-induced asthenozoospermia(AZS)using untargeted metabolomics,and to reveal its un-derlying mechanisms at the metabolite and pathway levels.Methods:Thirty 5-week-old male C57BL/6J mice were ran-domly divided into three groups:Normal,GTW(model),and BY1F.AZS model was established by daily oral administra-tion of GTW suspension(40 mg/kg,100 μl/d)for four consecutive weeks.The Normal group received an equal volume of de-ionized water.From weeks 5 to 8,the BY1F group additionally received daily oral gavage of BY1F granules[9.75 g/(kg·d),100 μl/d],composed of Barbary Wolfberry Fruit,Dodder Seed,Palmleaf Raspberry Fruit,Chinese Angelica,Plantain Seed,Milkvetch Root,Common Yam Rhizome,and Twotoothed Achyranthes Root,whereas,the other two groups continued receiving deionized water.Sperm concentration and motility in the epididymis were assessed microscopically.Metabolomic analysis was performed using serum samples with ultra-performance liquid chromatography-orbitrap mass spectrometry(UPLC-Orbitrap MS).Differential metabolites were identified through multivariate statistical analyses,including principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA),followed by KEGG pathway enrich-ment analysis.Results:Compared with the Normal group,sperm concentration and total motility in the epididymis of mice in the GTW group were significantly decreased.Both parameters markedly improved following treatment with BY1F,and the differences were statistically significant(P<0.05).Metabolomic analysis revealed a clear separation among the serum meta-bolic profiles of the three groups.A total of 13 shared differential metabolites were identified,among which betaine and phos-phatidylserine were significantly downregulated in the GTW group and subsequently restored following intervention.KEGG pathway enrichment analysis indicated that BY1F primarily modulates key pathways including"glycine,serine and threo-nine metabolism""ABC transporters""tryptophan metabolism"and"pyrimidine metabolism".Conclusion:BY1F effectively improves sperm quality in AZS mice,and its mechanism of action may be associated with the regulation of key metabolites such as betaine and phosphatidylserine,thereby influencing biological processes including amino acid metabolism,trans-membrane transport,and nucleic acid synthesis.关键词
弱精子症/不育1号方/代谢组学Key words
asthenozoospermia/Buyu No.1 Formula/metabolomics分类
医药卫生引用本文复制引用
Guan Wei,Song Xiaoyong,Du Zhimin,Gao Yuting..不育1号方改善弱精子症小鼠生殖功能的代谢组学机制[J].山西中医药大学学报,2025,26(11):1209-1215,7.基金项目
中央引导地方发展资金项目(YDZJSX2025D064) （YDZJSX2025D064）
山西省中医药科技专项科研课题(2024ZYY2C108) （2024ZYY2C108）
山西中医药大学基础与临床合作专项(2025PY-JL-29-01) （2025PY-JL-29-01）
