山西中医药大学学报2025，Vol.26Issue(11)：1221-1228,8.DOI:10.19763/j.cnki.2096-7403.2025.11.09
基于网络药理学探讨鸢尾素治疗肌少-骨质疏松症的作用机制
Discussion on the mechanism of irisin in treating osteosarcopenia based on network pharmacology
摘要
Abstract
Objective:To investigate the therapeutic effects and underlying mechanisms of irisin in treating osteosarco-penia(OS)using network pharmacology approaches.Methods:Literature mining and bioinformatics analyses were per-formed to identify potential targets of irisin.Disease-related targets for sarcopenia and osteoporosis were retrieved from the GeneCards,OMIM,and DisGeNET databases.Venn diagrams were generated using Venny 2.0 to identify overlapping targets between irisin and OS.A protein-protein interaction(PPI)network was constructed based on these intersecting targets,and core target genes were screened.Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were conducted using the Bioinformatics online platform to identify signal-ing pathways associated with irisin's therapeutic action in OS.Results:A total of 22 core targets of irisin and 366 disease-related targets for OS were identified,with 15 overlapping targets between them.PPI network analysis revealed key hub tar-gets including peroxisome proliferator-activated receptorγcoactivator 1-α(PPARGC1A),tumor necrosis factor(TNF),and RAC-α serine/threonine-protein kinase(Akt1).GO enrichment analysis indicated significant involvement in molecular functions(MF)such as cytokine activity and sequence-specific DNA binding,and biological processes(BP)including en-ergy homeostasis and negative regulation of adipocyte differentiation.KEGG pathway analysis highlighted several relevant signaling pathways,including alcoholic liver disease,atherosclerosis,longevity regulating pathway,insulin resistance,adipo-cytokine signaling pathway,and cellular senescence.Conclusion:The therapeutic mechanism of irisin in OS may be associ-ated with signaling pathways such as alcoholic liver disease,longevity regulation,insulin resistance,AMP-activated protein kinase(AMPK)signaling,adipocytokine signaling,and non-alcoholic fatty liver disease(NAFLD)signaling.Irisin likely exerts its beneficial effects by interacting extracellularly with key molecules including Akt kinase,vitamin D3 receptor(VDR),C-reactive protein(CRP),adiponectin(ADIPOQ),and fibronectin type III domain-containing protein 5(FNDC5),thereby promoting myoblast proliferation and osteoblast differentiation while inhibiting osteoclast formation,ulti-mately having a positive regulatory effect on osteosarcopenia.关键词
肌少-骨质疏松症/鸢尾素/网络药理学/作用机制Key words
osteosarcopenia/irisin/network pharmacology/mechanism of action分类
医药卫生引用本文复制引用
Li Guoqing,Xu Chao,Dong Dan,Wan Qingdong,Liang Bocheng,Liu Fenzhi..基于网络药理学探讨鸢尾素治疗肌少-骨质疏松症的作用机制[J].山西中医药大学学报,2025,26(11):1221-1228,8.基金项目
浙江省中医药科技计划(2023ZL058) （2023ZL058）
